Professional Research Assistant

Aurora, Arapahoe County
Jul 24, 2014

Posting Description: The major function of the Research Assistant will be performing molecular biology work including PCRs, Western Blotting, and immunohistochemistry, but also to be directly involved in murine studies, including exposure of mice to acute lung injury, myocardial ischemia, hepatic ischemia and kidney injury. In addition, the Professional Research Assistant will also be expected to participate in general laboratory tasks, such as tissue culture work, ordering of supplies etc. Moreover, active participation in the research work, including laboratory meetings of the Eltzschig laboratories and the Organ Protection Program are important aspects of the work. The Eltzschig laboratories and the Organ Protection Program, which represents an interdisciplinary research institute interest in understanding inflammatory diseases. The overall goal of this research institute is to identify endogenous pathways to dampen inflammation and use such pathways in a therapeutic effort. Dr. Eltzschig's specific research interest is in the development of novel therapeutic approaches towards organ ischemia, including kidney, heart and liver injury, and acute lung injury, induced by mechanical ventilation. Supervision Received: The incumbent will report to Dr. Holger, Eltzschig, MD, PhD, on a weekly basis and to Kelley Brodsky M.S. on a daily basis. The incumbent will receive close supervision by Kelley Brodsky and general directions by Dr. Eltzschig. Supervision Exercised: After initial training in molecular biology techniques and animal surgery, the Professional Research Assistant will also be involved in the training and supervision of more junior members of the laboratory, including medical students or junior professional research assistants. Examples of Work Performed: Molecular biology work including conventional reverse transcriptional polymerase chain reaction (RT-PCR) and real-time RT-PCR, protein chemistry, such as immunoprecipitation and Western blotting; histological techniques such as tissue sections, staining and immunohistochemistry. Tissue culture work, including feeding and splitting of cultured endothelial and epithelial cell lines, and work with siRNA silenced cell lines. Assisting with animal work, including hepatic, renal and myocardial ischemia, as well as ventilator induced lung injury in mice. Data gathering and documentation Assistance with data interpretation and analysis Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at CU Employee Services. The University of Colorado Denver is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees. The University of Colorado strongly supports the principle of diversity. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment. The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.

Minimum Qualifications: Bachelor's degree in a related field.

Required Competencies/Knowledge, Skills, and Abilities: Basic knowledge of biological systems and molecular approaches Good computer skills, including experience with Microsoft Office (particularly Word and Excel) Ability to quickly adapt to new challenges, willingness to learn, team spirit, good interaction skills.

Desired Qualifications: Master's degree in related field is preferred. Prior experience in research laboratory work.

Campus: Anschutz Medical Campus