Research Assistant I

Nashville, Davidson County
Jul 03, 2014


Project Duties:    
         Perform western blotting    
         Perform immunofluorescence staining procedures for cells and histological sections.
         Perform biochemical isolation studies on proteins.  
         Perform molecular cloning studies.    
         Perform yeast 2-hybrid studies.    
         Perform tissue culture procedures.    
         Must be willing and able to work with mice.    
         Experience with gel electrophoresis techniques a positive, although not required

Provide, under direct supervision, support for specified research projects; collect, assess, and communicate data/results. Assist in general laboratory operation and inventory.

Key Functions and Expected Performances:
  1. Under supervision, run experiments, assays, and record results
    1. Read and follow experimental protocols (May include animal handling, tissue culture, etc.)
    2. Compile and communicate results to supervisor
    3. Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogenics)
    4. Perform calculations involving molarity, % solutions, and serial dilutions
  2. Prepare and operate equipment in experimental procedures
    1. May include blood gas analyzer, centrifuge, pH meter, spectrophotometer, autoclave, scintillation counter, electrophoresis unit, analytical balance, etc.
    2. Maintain and repair equipment according to manufacturer and university guidelines.
  3. Ensure laboratory is prepared for experiments
    1. Maintain inventories,
    2. Maintain sterile conditions as required
    3. Prepare reagents
    4. Clean equipment.
    5. Prepare reagents

Basic Qualifications

Job requires Bachelor's and 0 years of experience or the equivalent.

Job Research

Primary Location TN-Nashville-Medical Research Bldg IV (MRB)

Organization Surgical Research 104708