Professional Research Asst

Aurora, Arapahoe County
Jun 18, 2014

Posting Description: Will be responsible for general laboratory supervision/maintenance and research work that involves plasmid cloning, PCR, Western blotting, mammalian cell culture, virus and viral vector preparation and use, molecular virology, including retroviral (HIV) life cycle analyses, luciferase and reverse transcriptase assays, flow cytometry, confocal microscopy, immunofluorescence. Site-specific gene editing with TAL effector nuclease and CRISPR/Cas systems are being pursued. In vivo studies involve innate immunity, transgenic approaches, and viral infection studies in mice and larger species. Duties and responsibilities: 1. Collaboration with PI or Senior PRA 2. Perform studies as related to research project 3. Diagnose and problem solve as it relates to the research project 4. Conduct screens and assays 5. Conduct literature searches 6. Preparation of tissues, cell cultures, blood samples, etc. for testing 7. Training of laboratory personnel in experimentation techniques 8. Measure and monitor gene expression (in mice) 9. Microscopic analysis 10. Animal care related to research procedures 11. Perform recombinant DNA work 12. Evaluation of receptor expression and virus susceptibility 13. Sequence cloned genes 14. Monitor cell interactions 15. Modify, test, verify and analyze research data 16. Prepare samples for analysis by various instrumentation 17. Perform experiments 18. Tabulate research data 19. Record results of experiments 20. Prepare research manuscripts and research presentations 21. Develop and maintain research databases Applications are accepted electronically at, refer to job posting RF01521. Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at The University of Colorado Denver is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees. The University of Colorado strongly supports the principle of diversity. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment. The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.

Minimum Qualifications: Bachelor's degree in molecular biology or related field.

Required Competencies/Knowledge, Skills, and Abilities: Molecular biology and mammalian cell culture training.

Desired Qualifications: Molecular biology experience, animal care and handling experiences. Helpful, can-do attitude. Taking ownership of projects. Reasonable writing skills. Specific virology experience, while a plus, is not a pre-requisite, as the laboratory will train the successful applicant in specific methods.

Campus: Anschutz Medical Campus