Boston, Suffolk County
Jan 15, 2014
Posting Title : RESEARCH TECHNICIAN/SR. RESEARCH TECHNICIAN - Microbiology - Basic Science/Medical
Requisition Number : 2376
City : Boston
State/Province : Massachusetts
Zip code : 02111
Job Description-Overview :

The Department of Molecular Biology and Microbiology is a Basic Science research department that carries out research on Microbiology, Molecular Biology, and Infectious Diseases. The department has teaching responsibilities in the Medical and Dental Schools. The Department also supports the Sackler graduate program in Molecular Microbiology.

The Research/Senior Research Technician will join a high-energy laboratory engaged in studies of viral mechanisms and be responsible for generating recombinant DNA constructs to support biochemical and cellular investigations. In addition, the applicant will be responsible for testing protein expression in eukaryotic or prokaryotic cells. Responsibilities will include:


  • Cloning genes of interest into expression vectors and vector map generation
  • DNA mutagenesis
  • Construct validation by sequencing
  • Meticulous record keeping, including organizing and maintaining laboratory construct library
  • Maintaining mammalian cell lines
  • Transfecting plasmids into cells, validating and optimizing protein expression
  • Protein detection using SDS-PAGE and western blot

Job Description - Requirements :

Basic Requirements: BS Degree in Biology or a related disciplineand 0-1 years for Research Technician and 1-3 years for a Senior Research Technician. Hands-on experience in molecular biology, e.g., PCR, cloning, mutagenesis, and familiarity with sterile technique are required. Proficiency in Microsoft Office, bioinformatic tools for primer design and sequence analysis, English, and good communication skills are required.

Preferred Requirements: Preference will be given to candidates with extensive experience with recombinant DNA technology. Experience with mammalian cells is preferred but not required.

Please Apply with Job Requisition #2376

Tufts University is an AA/EO employer and actively seeks candidates from diverse backgrounds.