Research Technician in cryogenic electron microscopy - Part-Time

Job No: 531271
Work Type: Staff Part-Time
Location: Palm Beach
Categories: Biology/Life Science
Department: 37043010 - SR-IS&CB-IZARD LAB

Job Description


Classification Title:

Research Technician

Job Description:

A part-time Research Technician position is available in Professor Tina Izard's cell adhesion laboratory.

The laboratory collaborates with several other laboratories on campus including exciting collaborative and currently active projects with Professors Kirill Martenmyanov (www.science.org/doi/10.1126/science.abl4732), Kendall Nettles (https://doi.org/10.1073/pnas.210165711), Susana Valente (https://pubmed.ncbi.nlm.nih.gov/30723126), and Luiz Carvalho (https://scripps.ufl.edu/profile/carvalho-luiz-pedro/).

Thus, there are several exciting opportunities for a variety of very interesting projects beyond structure-function studies of cytoskeletal proteins that control signaling downstream of adherens junctions and focal adhesions with implications in cancer and heart diseases and more recently
structure-function studies of novel Mycobacterium tuberculosis targets in collaboration with Professor Luiz Carvalho (https://scripps.ufl.edu/profile/carvalho-luiz-pedro/).

The laboratory is recognized as a world leader and provides outstanding training in protein biochemistry and structural, molecular, and cellular biology, in a highly dynamic and team-oriented research program.

Successful candidates will work in a cutting-edge research environment with new state-of-the-art facilities. The laboratory has outstanding facilities for cell biology, molecular biology, protein expression, purification, biochemistry, and cryogenic electron microscopy, and protein crystallography. The laboratory has access to many outstanding Cores that facilitate basic research and drug development. The unique infrastructure includes the application of unique chemical, genomic, proteomic, informatics, and high-throughput screening and engineering tools for the discovery of novel biomolecules.

Professor Tina Izard is a board member of the South East Regional Collaborative Access Team (SER-CAT) that constructed two beam lines at the Advanced Photon Source (APS) at Argonne National Laboratory. The insertion device and bending magnet beam lines are available for all the synchrotron-related data collection needs for the post-doctoral associates.

Professor Tina Izard recently installed the first 300 kV cryo Atomic Resolution Microscope (cryoARM300) in the Americas (https://www.youtube.com/watch?v=xdVxAN8xy1w&feature=youtu.be) and has plenty of microscope time dedicated to the post-doctoral associate. This state-of-the-art transmission electron microscope is a 300 kV high-resolution microscope capable of carrying out cryogenic-electron microscopy studies by providing atomic resolution for structural studies involving single particle analyses. The transmission electron microscope is equipped with a new and improved Gatan K3 direct electron detector camera with better DQE, larger field of view (24 Megapixels - 5,760 x 4,092) and faster (3.75 times that of K2 camera). The direct detection devices are exquisitely sensitive, produce high signal-to-noise images, and are ideal for high-resolution studies.

Professor Izard designed and installed a HighFlux X-ray diffraction system, specifically a Rigaku MicroMax-007 HF, a second-generation microfocus rotating anode generator together with a mar345 Image Plate detector. An Osmic VariMax optics system and the Proteros Free Mounting System can be used to control the humidity and to optimize diffraction characteristics, including resolution, mosaicity, and anisotropy, as well as reduced X-ray background.

Expected Salary:

Commensurate with experience.

Minimum Requirements:

A bachelors degree in an appropriate area of specialization or an equivalent combination of education and experience.

Preferred Qualifications:

Applicants with experience in either protein biochemistry or cryogenic electron microscopy are preferred.

Special Instructions to Applicants:

In order to be considered, please upload your resume and cover letter.

Please provide names and email addresses of three to five references as part of the application process. When ready, the hiring department will contact the listed references via email requesting their reference letters to be uploaded directly to the application website.

This position is eligible for veteran's preference. If you are claiming veteran's preference, please upload a copy of your DD 214 Member Copy 4 with your application for consideration. See the Qualified Positions link on our applicant FAQs page for a list of eligible positions.

For any inquiries, you may contact Dr. T. Izard, at t.izard@ufl.edu.

This position is open until filled. Application review will begin May 3, 2024.

This is a time-limited position.

This position is part-time and will work Monday through Friday. A set schedule will be discussed during the interview process.

Health Assessment Required:
No.



Applications Close: Open until filled

To apply, visit https://explore.jobs.ufl.edu/en-us/job/531271/research-technician-in-cryogenic-electron-microscopy-parttime

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