This position is responsible for duties in support of lab research projects in metabolism and gene transcription.  Range of duties includes but is not limited to: managing a mouse colony; performing molecular biology techniques, immunology techniques; animal model research; data collection and analysis; assisting with lab maintenance such as sterilization of materials and preparation of solutions; maintaining adequate inventory of materials and equipment to ensure supplies for experimental needs. 

ESSENTIAL FUNCTIONS

• (70%) Research Support
• Manage a mouse colony, including mouse handling, genotyping, and record keeping.
• Cell culture.
• Molecular biology techniques, including RNA and DNA extraction and analysis, cloning (restriction enzyme digestion, ligation, minipreps), PCR and RT-PCR, Western blot, gel electrophoresis, RNA-seq and ChIP-seq, CRISPR/Cas9 based genome editing.
• Metabolic analysis of mouse models. Isolation of tissue and serum from mice. physiological analysis of glucose and fat metabolism: glucose tolerance test, insulin tolerance test, metabolic cage analysis. Isolation of stromal vascular fraction and in vitro differentiation into adipocytes.

• (30%) Laboratory Support
• Provides assistance to the lab manager to ensure the operation of essential equipment, making sure adequate supplies are available for routine experimental procedures, etc.
• Maintain a clean, orderly work area within the laboratory.
• Perform routine maintenance and make minor repairs to simple laboratory apparatus and instruments.
• Assist in the set-up and operation of simple laboratory apparatus and instruments; read and record instrument data; tabulate data; keep laboratory records.
• Perform an inventory of chemicals and supplies; order laboratory supplies and stock laboratory areas as required.
• Operate sterilization equipment ensuring appropriate conditions for the material being processed.
• Prepares various solutions and/or media according to established protocols.

EXPERIENCE

Required:

• Basic lab experience with relevant degree.

Preferred:

• Demonstrated experience and application of molecular biology knowledge preferably in biochemistry and cell culture.
• Experience with data analysis.
• Experience working with mice.
• Experience as a lab technician or research assistant, or equivalent combination of education and experience.    
• Experience with general lab maintenance, including cleaning, stocking supply inventory, and ensuring the maintenance of a sterile environment. 

EDUCATION

Required

• Bachelor's degree in Biological Sciences or related scientific discipline required. Bachelor's degrees in a different discipline and a minimum of 2 years of experience in a scientific role will be considered in lieu.

Preferred

• Master’s degree in biological sciences or related field.

The expected pay range for this position is $18.03 - $21.00 per hour.
Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs.

SPECIAL CONDITIONS OF EMPLOYMENT

• Must be willing to work in an animal-related research environment.
• Satisfactory completion of the Institute’s background investigation.
• Willing to sign a confidentiality agreement

PHYSICAL REQUIREMENTS/MENTAL ACTIVITIES/ENVIRONMENTAL CONDITIONS

The incumbent in this position will be constantly reading, and working indoors while frequently reasoning, analyzing, touching/feeling, talking, and standing.

The Salk Institute is an internationally renowned research institution that values diversity, equity, and inclusion. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces diverse perspectives and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.