Under the direction of the PI, the Research Associate III performs a high level of independent research within the area of NeuroHIV and Gene-Editing. The Research Associate III will coordinate experimental procedures, and analyze data generated from experiments performed. The Research Associate III is also expected to train laboratory personnel (including research assistants, student) in research methods, procedures, equipment, troubleshoot experiments when needed.Responsibilities include: Culture plant cell cultures i.e. protoplasts from plants and propagate into Callus. Apply CRISPR techniques in editing of plant cell cultures and determine efficient Excision with molecular biological tools; performing common molecular, biochemical, and cellular methodologies (Western blotting, ELISA, PCR and qPCR); flow cytometry, immunohistochemistry/laser confocal microscopy, permeability assays, and immunological assays; assist in grant/manuscript writing. Performs other related duties as assigned.Required Education & Experience:
Bachelor's Degree and three years of research experience. An equivalent combination of education and experience may be considered. Required Skills & Abilities:
*Practical experience in flow cytometry, intracellular cytokine staining, immunohistochemistry, and immunological assays and molecular biological assays
*Demonstrated experience/proficiency in experimental methodologies
*Demonstrated experience managing and handling lab animals
*Strong attention to detail
*Ability to work independently with high level of personal and professional drive
*Working knowledge of applicable computer software programs related to the researchPreferred:
* Master's DegreeThis position requires a background check.
This is a grant funded position.Temple University values diversity and is committed to equal opportunity for all persons regardless of age, color, disability, ethnicity, marital status, national origin, race, religion, sex, sexual orientation, veteran status or any other status protected by law.In accordance with the City of Philadelphia's COVID-19 mandate, all Temple University students, faculty, staff, and contractors must be fully vaccinated before receiving authorization to work unless they have a medical or religious exemption approved by the University. An individual is considered fully vaccinated two (2) weeks following the second dose of the Pfizer or Moderna vaccine or two (2) weeks following a single dose of the Johnson & Johnson vaccine. Individuals who do not meet the University's vaccination or exemption requirement will be prohibited from working until proof of full vaccination or an exemption is requested and approved. For more information, visit the University's Vaccines web page at https://www.temple.edu/coronavirus/vaccines.Compliance Statement: In the performance of their functions as detailed in the position description employees have an obligation to avoid ethical, legal, financial and other conflicts of interest to ensure that their actions and outside activities do not conflict with their primary employment responsibilities at the institution. Employees are also expected to understand and be in compliance with applicable laws, University and employment policies and regulations, including NCAA regulations for areas and departments which their essential functions cause them to interact.To obtain additional information about Temple University please visit our website at www.temple.edu. Temple University's Annual Security and Fire Safety Report contains statistics, policies, and procedures related to campus safety and can be found at: https://safety.temple.edu/reports-logs/annual-security-reportYou may request a copy of the report by calling Temple University’s Campus Safety Services at 215-204-7900.
Primary Location: Location INSIDE of PA
Shift: Day Job
Employee Status: Regular