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Life Science Research Professional 1

Employer
Stanford University
Location
Stanford

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Administrative Jobs
Academic Affairs, Research Staff & Technicians
Employment Type
Full Time
Institution Type
Four-Year Institution

Job Details



Life Science Research Professional 1
School of Medicine, Stanford, California, United States

Schedule: Full-time
Job Code: 4945
Employee Status: Regular
Grade: E
Requisition ID: 94554

The Department of Pathology at Stanford University is seeking a Life Science Research Professional to join Ansu Satpathy's Laboratory ideally in summer 2022. The candidate will apply high throughput gene editing technologies in human immune cells to study and develop cancer immunotherapies.

Lab Overview: The Satpathy Lab studies fundamental principles of the immune system in health, infection, and cancer (Parker et al, Cell 2020; Flynn et al, Cell 2021). The lab particularly focuses on single-cell genomic and multi-omic technologies (Yost et al, Nature Medicine 2019; Rubin et al, Cell 2019) to study immunological mechanisms of disease directly in samples isolated from patients. For more information about our multidisciplinary research laboratory, see https://satpathylab.com/.

We are recruiting a Life Science Research Professional to join a new research group within the lab focused on using gene editing and high-dimensional screening in primary human immune cells to parse the biology of cancer-immune interactions and develop new immune cell-based cancer therapies. This gene editing focused group is located at the Stanford Blood Center (3373 Hillview Ave), a short bike ride from the main campus, and will be led by Dr. Theo Roth, MD, PhD. The group's work involves synthetic re-writing of human T cell genomes using non-viral gene editing (Roth et al, Nature 2018) in high throughput arrayed and pooled genetic screens (Roth et al, Cell 2020).

About the Position: Working as part of a multidisciplinary group, the LSRP will design and construct DNA sequences for cellular engineering experiments, isolate and culture primary human immune cells, and perform functional analysis of engineered cells via flow cytometry and cancer co-culture assays. Additional major responsibilities will include next-generation sequencing library production and analysis. For exceptionally skilled applicants, the LSRP's role can be extended to include the design, completion, and analysis of cellular editing experiments and pooled screens. This position represents an excellent opportunity to gain exposure to basic laboratory skills as well as advanced genetics and genome engineering technologies in a dynamic research environment.

As an organization that receives federal funding, Stanford University has a COVID-19 vaccination requirement that will apply to all university employees, including those working remotely in the United States and applicable subcontractors. To learn more about COVID policies and guidelines for Stanford University Staff, please visit https://cardinalatwork.stanford.edu/working-stanford/covid-19/interim-policies/covid-19-surveillance-testing-policy" delay="150" href="https://apptrkr.com/get_redirect.php?id=3089324&targetURL=https://cardinalatwork.stanford.edu/working-stanford/covid-19/interim-policies/covid-19-surveillance-testing-policy" style="box-sizing:inherit; color:rgba(var(--sk_highlight,18,100,163),1); font-variant-ligatures:common-ligatures; text-decoration:underline" target="_blank">https://cardinalatwork.stanford.edu/working-stanford/covid-19/interim-policies/covid-19-surveillance-testing-policy.

Duties include:
  • Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
  • Independently conduct experiments; maintain detailed records of experiments and outcomes.
  • Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
  • Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
  • Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
  • Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
  • Assist with orientation and training of new staff or students on lab procedures or techniques.

*- Other duties may also be assigned

DESIRED QUALIFICATIONS:

  • Bachelor's degree in life sciences or a related area;

  • Knowledge and experience in any of the following fields: immunology, molecular biology, cell biology, or related biology and bio-engineering fields.

  • Familiarity with experimental protocols (“wet-lab”) in immunology/molecular and cellular biology.

  • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including DNA and RNA isolation, PCR, DNA assembly, bacterial transformation, eukaryotic cell culture, etc.)

    • Prior experience in the design and assembly of DNA constructs

    • Prior experience with primary human immune cell cultures, including isolation and activation

    • Prior experience with DNA sequencing technologies, including Sanger and NGS

  • Self-motivated, possessing strong communication skills, and the ability and desire to perform as part of a team.

EDUCATION & EXPERIENCE (REQUIRED):

Bachelor's degree in related scientific field.

KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):

  • General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.

  • Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.

  • Ability to maintain detailed records of experiments and outcomes.

  • General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.

  • Ability to work under deadlines with general guidance.

  • Excellent organizational skills and demonstrated ability to accurately complete detailed work.

CERTIFICATIONS & LICENSES:

None

PHYSICAL REQUIREMENTS*:

  • Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.

  • Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.

  • Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.

  • Must have correctible vision to perform duties of the job.

  • Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.

  • Ability to lift heavy objects weighing up to 50 pounds.

  • Ability to work in a dusty, dirty, and odorous environment.

  • Position may require repetitive motion.

*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

WORKING CONDITIONS:

  • May require working in close proximity to blood borne pathogens.

  • May require work in an environment where animals are used for teaching and research.

  • Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.

  • Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.

  • May require extended or unusual work hours based on research requirements and business needs.


To be considered for this position please visit our web site and apply on line at the following link: https://apptrkr.com/3089324

Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.







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