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RESEARCH TECHNICIAN II

Employer
Duke University
Location
Duke Human Vaccine Institute - Admin

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Administrative Jobs
Academic Affairs, Research Staff & Technicians
Employment Type
Full Time
Institution Type
Four-Year Institution
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Job Details

This Position is Fully Grant Funded

School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

POSITION OVERVIEW

The Duke Human Vaccine Institute (DHVI) continues to lead cutting edge vaccine research against infectious diseases that impact global health. Basic science discoveries made using a variety of human and pathogen-derived proteins designed, expressed, and purified by the highly collaborative DHVI have contributed to the identification of vaccine and therapeutic candidates currently being produced in Good Manufacturing Practice facilities for early phase clinical trials.

The Saunders laboratory aims to understand the immunology of HIV-1 antibodies and the molecular biology of their interaction with HIV-1 envelope (Env) glycoprotein. Our overall goal is to develop protective antibody-based vaccines; therefore, the laboratory has two sections – immunogen design and antibody repertoire analysis. Proteins derived from HIV envelope are designed and expressed as candidate vaccine immunogens for preclinical trials. Additionally, the proteins are used to probe B cells in humans and rhesus macaques in an effort to identify antibody responses stimulated by these immunogens. Lastly, we use in vitro assays to functionally characterize the identified antibodies. Thus, our overall efforts are aimed at designing an effective HIV vaccine to halt the AIDS pandemic.

We are seeking a motivated and hardworking Research Technician II to support our vaccine development research. The primary responsibilities will include the production of HIV envelopes and nanoparticles, and recombinant antibodies.

Responsibilities

  • Apply aseptic cell culture techniques to express candidate HIV vaccine immunogens in eukaryotic cells through transient transfection
  • Perform the following experimental activities:Large scale antibody, HIV-1 envelope and nanoparticle, and host protein production and purificationAnalysis of recombinant antibodies and HIV-1 envelopes using biochemical and immunological techniques, such as FPLC, SDS-PAGE, Western blots, ELISA, and biolayer interferometry
  • Molecular biology assays such as PCR, site-directed mutagenesis, DNA preparation
  • Sanger sequencing of plasmid DNAHIV-1 envelope and antibody sequence analysisBiotinylation and fluorescent labeling of recombinant proteinsFlow cytometry analysis of fluorescent proteins
  • Perform routine lab maintenance, cleaning, organizing, and restocking supplies
  • Includes keeping lab equipment, chemicals, reagents, and supplies in excellent order, and managing protein inventories
  • Understand and comply with all safety standards and procedures to ensure a safe working environment for all laboratory staff
  • Closely follow all standard operating procedures
  • Compile, analyze, document, and draw tentative conclusions from experimental results
  • Confer with the principal investigator/manager to review work assignments and develop plans for research experiments
  • Track work and compliantly order materials and reagents associated with numerous projects under multiple grants
  • Collaborate with other staff and perform other duties as requested by the Principal Investigator or senior lab staff
  • Required Qualifications at this Level

    • Education/Training: Work generally requires a bachelor's degree in biology or other directly related scientific field.
    • Experience: None required beyond above education/training requirement.

    Preferred Education, Skills, & Experience Requirements

    • Some previous lab experience is highly desired
    • Demonstrated ability to work both independently and within a team, and to troubleshoot and resolve problems, is required
    • Must have excellent oral and written communication skills
    • Must be detail-oriented and well organized

    Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

    Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

    Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

    Organization

    Read our Diversity Profile History

    Duke University was created in 1924 by James Buchanan Duke as a memorial to his father, Washington Duke. The Dukes, a Durham family that built a worldwide financial empire in the manufacture of tobacco products and developed electricity production in the Carolinas, long had been interested in Trinity College. Trinity traced its roots to 1838 in nearby Randolph County when local Methodist and Quaker communities opened Union Institute. The school, then named Trinity College, moved to Durham in 1892, where Benjamin Newton Duke served as a primary benefactor and link with the Duke family until his death in 1929. In December 1924, the provisions of indenture by Benjamin’s brother, James B. Duke, created the family philanthropic foundation, The Duke Endowment, which provided for the expansion of Trinity College into Duke University.Duke Campus

    As a result of the Duke gift, Trinity underwent both physical and academic expansion. The original Durham campus became known as East Campus when it was rebuilt in stately Georgian architecture. West Campus, Gothic in style and dominated by the soaring 210-foot tower of Duke Chapel, opened in 1930. East Campus served as home of the Woman's College of Duke University until 1972, when the men's and women's undergraduate colleges merged. Both men and women undergraduates now enroll in either the Trinity College of Arts & Sciences or the Pratt School of Engineering. In 1995, East Campus became the home for all first-year students.

    Duke maintains a historic affiliation with the United Methodist Church.

    Home of the Blue Devils, Duke University has about 13,000 undergraduate and graduate students and a world-class faculty helping to expand the frontiers of knowledge. The university has a strong commitment to applying knowledge in service to society, both near its North Carolina campus and around the world.

    Mission Statement

    Duke Science"James B. Duke's founding Indenture of Duke University directed the members of the University to 'provide real leadership in the educational world' by choosing individuals of 'outstanding character, ability, and vision' to serve as its officers, trustees and faculty; by carefully selecting students of 'character, determination and application;' and by pursuing those areas of teaching and scholarship that would 'most help to develop our resources, increase our wisdom, and promote human happiness.'

    “To these ends, the mission of Duke University is to provide a superior liberal education to undergraduate students, attending not only to their intellectual growth but also to their development as adults committed to high ethical standards and full participation as leaders in their communities; to prepare future members of the learned professions for lives of skilled and ethical service by providing excellent graduate and professional education; to advance the frontiers of knowledge and contribute boldly to the international community of scholarship; to promote an intellectual environment built on a commitment to free and open inquiry; to help those who suffer, cure disease, and promote health, through sophisticated medical research and thoughtful patient care; to provide wide ranging educational opportunities, on and beyond our campuses, for traditional students, active professionals and life-long learners using the power of information technologies; and to promote a deep appreciation for the range of human difference and potential, a sense of the obligations and rewards of citizenship, and a commitment to learning, freedom and truth.Duke Meeting

     “By pursuing these objectives with vision and integrity, Duke University seeks to engage the mind, elevate the spirit, and stimulate the best effort of all who are associated with the University; to contribute in diverse ways to the local community, the state, the nation and the world; and to attain and maintain a place of real leadership in all that we do.”

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