DescriptionUniversity of Colorado Denver l Anschutz Medical CampusSchool of Medicine, Department of SugeryDivision of UrologyPost-Doctoral FellowPosition #00785308– Requisition #23334
* Applications are accepted electronically ONLY at www.cu.edu/cu-careers
The University of Colorado has a requirement for COVID-19 vaccinations and full completion thereof by 9/1/21 or upon start date. Information regarding this requirement, and exemptions can be found at:Anschutz: https://www.ucdenver.edu/docs/librariesprovider284/default-document-library/3000-general-admission/3012---covid-19-vaccination-requirement-and-compliance.pdf?sfvrsn=4e9df3ba_2Denver: https://www.ucdenver.edu/coronavirusExemptions vary by campus location/department. Campus/Unit-Specific Exemptions:
- Anschutz Campus – Exemptions are allowed for medical or religious reasons.
- Denver Campus - Exemptions are allowed for medical, religious, or personal reasons.
- Consolidated/Central Services Administration – Will follow Anschutz policy on exemptions.
The University of Colorado Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment. We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.The Department of Surgery, Division of Urology has an opening for a full-time (1.0 FTE) Post-Doctoral Fellow. The Incumbent will be responsible for experiements proposed in R01 DK129260-01 studying the roles of GFAP-positive glia in bladder physiology and diseases. Research projects utilize mouse models for urinary dysfunction and chronic pelvic pain. Genetic mouse models and adeno-associated viral vectors (AAVs)-medicated pharmacogenetic manipulation will be used to control neuron and glia signaling in vivo.
Behavioral, physiological, and electrophysiological recordings for bladder functions in vivo
and in situ
. Ganglionic implants and primary cell culture will be used to study the molecular mechanisms underlying glia-neuron interaction in perhipheral ganglia controlling bladder function in vitro
. Standard techniques studying cell-cell interaction in situ
include confocal microscopy, patch-clamp recordings, and calcium imaging. Specific Position Duties:
Investigating the neuromodulatory roles of satellite glial cells in the peripheral ganglia receiving bladder sensory signals following their GPCR activation using cultured peripheral ganglia.Evaluating the functional relevance of sensory satellite glia in the pathogenesis and therapeutics for bladder hypersensitivity and chronic pelvic pain.Characterizing the translatome changes in peripheral sensory neuron and satellite glia signaling in mouse models of chronic pelvic pain and bladder overactivity.Utilizing pharmacogenetic approach (DREADDs) to control the excitability/activity of bladder nociceptive neurons and nearby glial cells in vivo.Design cell-specific AAVs and optimize the protocol for tissue-specific transduction using AAVs in selected cell type in the peripheral nervous system.Based on the research question, experimental methods might include nucleic acid and protein isolation, RT-PCR and qPCR, Western blotting, AAV injections, tissue dissection/culture, perfusion and immunohistochemistry, confocal microscopy, electrophysiology, in vivo behavior and physiological recordings, mouse breeding and colony management.Actively participating and presenting in weekly lab meetings, and weekly progress meetings with the PI.Prepare data for internal presentations (to the research group, the Department, etc) and external presentations (such as in conference meetings) as well as publications.Salary and Benefits:
The approximate salary range for this position is based on NIH salary levels at $53,760 - $65,292 and is commensurate with experience.The salary of the finalist(s) selected for this role will be set based on a variety of factors, including but not limited to, internal equity, experience, education, specialty and training.The above salary range (or hiring range) represents the University’s good faith and reasonable estimate of the range of possible compensation at the time of posting. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services
.University of Colorado Denver | Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background checks for all new employees prior to their employment.The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave, health plans and retirement contributions that add to your bottom line.Benefits: https://www.cu.edu/employee-services/benefits
.Total Compensation Calculator: https://www.cu.edu/employee-services/total-compensationDiversity and Equity:
Please contact [email protected]
for information on disability accommodationsOffice of Equity: https://www1.ucdenver.edu/offices/equity
The University of Colorado Denver | Anschutz Medical Campus is committed to recruiting and supporting a diverse student body, faculty and administrative staff. The university strives to promote a culture of inclusiveness, respect, communication and understanding. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.The University of Colorado Denver | Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees.
Ph.D. in relevant background such as neuroscience, biochemistry, biophysics, cell biology, and behavioral sciencesAt least 3 years of laboratory research experienceTraining in electrophysiology (in vivo or in situ).First author publication(s) in peer-reviewed biomedical journals.Applicants must meet minimum qualifications at time of hirePreferred Qualifications:
Research eperiences in GFAP+ glia or neuron-glia interactionsExperiences in patch-clamping electrophysiology and/or confocal/2P microscopy.Experience in mutagensis, AAVs, qRT-PCR, Western Blot; cell or tissue culture, generation or maintainence of transgenic/KO colonies.Experience in behavioral or physiological recordings in vivo.Experience in bioinformatic studiesJob Category:
Oct 21, 2021Unposting Date: