UCSD Layoff from Career Appointment: Apply by 10/22/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 11/2/22. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This position will remain open until a candidate has been successfully identified.
Provides hands-on technical support in electron and light microscopy methods. Collaborates with researchers from several laboratories, aids the research group and independently conducts laboratory investigations at NCMIR. Performs and develops both specimen preparation and sectioning techniques in the areas of high resolution transmission and intermediate high voltage electron microscopy, immunofluorescence, immunocytochemistry, protein labeling, serial sectioning, computer-aided 3-dimensional reconstruction, tomographic reconstruction within thick biological specimen. Develops, modifies and implements freeze fracture techniques, 2-photon imaging and confocal microscopy. Obtains large scale brain mapping data and examines and acquires high quality images of specimens for both data analysis and publication.Teaches and trains undergraduate students, graduate students, post-docs and collaborators on equipment and laboratory procedures used in confocal and electron microscopy.
Theoretical knowledge of histology, histochemistry, molecular and cellular biology as well as chemistry and biochemistry.
Skill in providing individual instruction on the use of the transmission electron microscope, confocal microscope, and peripheral support of these instruments.
Familiarity and skill with software for processing and creating two-dimensional and three-dimensional images, especially for tomography.
Ability to understand and evaluate scientific literature. This includes all aspects of the research project and to interpret results for scientific publications.
Excellent written and oral communication skills and ability to juggle multiple projects at once with good organization skills.
Experience in the production of high quality vibratome sections for confocal microscopy. Familiarity with neuroanatomical landmarks and cellular components in brain slices.
Experience in the production of high quality light images and both electron microscopy, CCD images, and electron micrographs.
Experience and skill with transmitted light microscopy, laser scanning confocal, transmission electron microscopy, and high voltage electron microscopy. Ability to increase skill with multi-photon microscopy.
Experience to produce high quality thin and semi-thin sections for electron microscopy with glass and diamond knife. Including serial sectioning of thick sections for ultra-high electron microscopy.
Understanding of theory and practice of chemical tissue fixation, high pressure freeze fixation and freeze fracture for localization procedures and ultrastructural procedures used in transmission electron microscopy, confocal microscopy, and light microscopy.
Understanding both the theory and practice of various cellular localization labeling techniques for light, confocal, and transmission electron microscopy. Experience with certain cellular localization procedures. Ability to learn new labeling techniques.
Experience with high resolution localization labeling techniques (such as colloidal gold etc.) for electron microscopy. Ability to learn new labeling techniques.
- Knowledge of graphing software (e.g., GraphPad prism) and basic understanding of statistical analyses is a plus.
- Basic familiarity with Linux operating system.
- Employment is subject to a criminal background check.