Post-Doctoral Fellow

Employer
University of Colorado Anschutz Medical Campus
Location
Denver, CO
Closing date
Oct 22, 2021

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Job Details

Description
UNIVERSITY OF COLORADO ANSCHUTZ MEDICAL CAMPUS

Department of Biochemistry and Molecular Genetics

School of Medicine

Postdoctoral Fellow/Trainee

Position Classification: Research Faculty

Research area: Subcellular RNA Localization

The University of Colorado Denver l Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment.  We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.

Overall Description of Responsibility or Nature of Work

This position reports directly to Dr. Matthew Taliaferro and Dr. Chad Pearson and is responsible for directing and performing research related to subcellular RNA localization to centrosomes.  The goal of this research is to understand the mechanism by which individual RNA molecules are transported to centrosomes as well as consequences of RNA mislocalization.

The spatial organization of proteins within cells is critical to their proper function.  This spatial arrangement can be achieved by transporting fully translated proteins to their required destination. Alternatively, it can be done by transporting the instructions for making the protein, that is, an mRNA, to the desired location followed by on site protein production.  It has been recently observed that centrosomes are associated with a distinct localized transcriptome.  However, the nature of these RNA molecules, how they are trafficked to the centrosome, and the consequences of their mislocalization are all generally unclear.

Active areas of research include (1) determining the identities of centrosome-proximal RNAs and their dynamic localization across the cell cycle, (2) high-throughput screening to identify RNA sequences with the ability to drive localization to centrosomes, and (3) identifying cellular phenotypes associated with RNA mislocalization.

Research will involve wet-lab experimental procedures and computational analyses of high-throughput sequencing data.  Applicants with familiarity with only one of these techniques and the desire to increase experience with the other are encouraged to apply.

For additional information about the laboratory or to see recent publications, visit www.taliaferrolab.com and www.thepearsonlab.com.  Applications will be reviewed immediately and the search will remain open until the positions are filled.  Interested applicants may also send an email to [email protected] and [email protected] for more information.

Candidates will be expected to plan and execute independent work, write and submit fellowship applications (if applicable), assist in the writing and publication of experimental results, and contribute positively towards the atmosphere and culture of the lab.

Specific Position Duties with Percentages of Time

80%

The overall goal of the work is to understand how mRNA molecules are trafficked to cellular locations, specifically to centrosomes.  The techniques used to study this phenomenon include mammalian cell culture, nucleic acid purification and sequencing, computational biology, single molecule RNA FISH, genome engineering, and molecular genetics.  Experiments are to be designed and conducted to investigate the mechanism by which RNA-binding proteins (RBPs) that regulate RNA localization recognize their targets and mediate their transport.  These experiments will involve the culturing of mammalian cells, cell fractionation, high-throughput sequencing library preparation, and the bioinformatic analysis of the resulting sequencing data.  Familiarity with standard bioinformatic tools and the ability to design and write custom software (preferably in Python and/or R) are preferred but not required.  However, the candidate will be expected to put effort towards gaining these skills during the course of their research.  Efforts will also be directed at confirming and extending findings from genome-wide experiments using single molecule RNA FISH and structure-function experiments using RNA reporters.

20%

The candidate will also assist in the preparation of manuscripts to publish their work, the preparation of grant applications, both internal and external, and the preparation of scientific data for presentation at various meetings and conferences.  The candidate will also mentor junior scientists in the lab in their scientific development.

The candidate will contribute to research involving one or more of the following:

  • Molecular biology and biochemistry experiments
  • Cell culture involving immortalized mammalian cell lines and primary mouse tissues
  • RNA isolation and purification
  • Generation of next-generation sequencing libraries
  • Crosslinking and immunoprecipitation followed by high-throughput sequencing (CLIP-seq)
  • Genome engineering using CRISPR-Cas9, Cre/lox, and FRT
  • Fluorescence deconvolution microscopy and single molecule RNA FISH
Salary and Benefits: 

The salary range (or hiring range) has been established at $53,760 - $65,292

The salary of the finalist(s) selected for this role will be set based on a variety of factors, including but not limited to, internal equity, experience, education, specialty and training.

The above salary range (or hiring range) represents the University’s good faith and reasonable estimate of the range of possible compensation at the time of posting.

The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.

University of Colorado Denver | Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background checks for all new employees prior to their employment.

The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.

Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave, health plans and retirement contributions that add to your bottom line.

Benefits: https://www.cu.edu/employee-services/benefits.

Total Compensation Calculator: https://www.cu.edu/employee-services/total-compensation

Diversity and Equity:

Please contact [email protected] for information on disability accommodations

The University of Colorado Denver | Anschutz Medical Campus is committed to recruiting and supporting a diverse student body, faculty and administrative staff. The university strives to promote a culture of inclusiveness, respect, communication and understanding. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.

The University of Colorado Denver | Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees.



Qualifications
Minimum Qualifications: 

  • PhD in biology or closely related field
  • Notated peer-reviewed publications in internationally recognized journals
  • Experience in molecular biology and cell culture techniques
  • Experience in computational biology techniques, including the generation of custom software, is preferred but not required
Knowledge, Skills, and Abilities:

  • Excellent communication skills
  • The successful candidate is expected to be highly motivated, have ample scientific curiosity, have strong interpersonal skills, and be able to contribute in a positive manner to the collegial atmosphere of the laboratory and the department.  The candidate will be expected to direct independent research, mentor more junior lab members, and apply for independent fellowship funding.
Applicants must meet minimum qualifications at the time of hire



Job Category: Faculty
Primary Location: Aurora
Schedule: Full-time
Posting Date: Jul 12, 2021
Unposting Date: Ongoing

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