School of Medicine:
Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.
Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
Post Doc for Gentzon Hall, MD Lab in DMPI, Carmichael Bldg, Division of Nephrology, Department of Medicine
The Hall lab is situated in the Duke Molecular Physiology Institute at the Duke University School of Medicine. Dr. Hall’s lab is focused on elucidating the mechanisms of podocyte injury and apoptosis in familial nephrotic syndrome. The lab primarily studies the pathobiology of focal segmental glomerulosclerosis, a protein-wasting kidney disease that disproportionately affects African-Americans.
The lab is seeking a highly motivated postdoctoral fellow to lead the investigation of disease-causing genetic mutations in key podocyte genes known to cause or contribute to inherited FSGS. Relevant experience includes:
a.) in vivo modeling in zebrafish (i.e. husbandry and colony maintenance, CRISPR and morpholino injections, tissue sectioning and EM imaging of the pronephric structures, etc.)
b.) optimization of biochemical assays (i.e. luciferase, chromatin immunoprecipitation, and
c.) PCR/qPCR, tissue culture (i.e. maintenance of immortalized cell lines), FACS sorting and analyses
d.) microscopy (i.e. confocal, light and fluorescence)
e.) live cell imaging assays
f.) statistical analyses and data management
g.) scientific writing and presentation
The candidate will benefit from working in a multi-lab environment that includes strong “omics” core labs and will be expected to collaborate across labs to complete the project objectives.
The nature of work will include: urgent tissue culture, luciferase assays, apoptosis studies, FACS analyses and qPCR will resume when work restrictions are lifted.
The post-doc will split their time between the on-campus zebrafish facility and the Duke Molecular Physiology Institute. The post-doc will have a work-station with a computer and a phone. They will perform wet-lab activities in Bay 51-112 at a dedicated bench and have access to Dr. Hall's dedicated tissue culture room in the same Bay. The PCR, qPCR and automated plate-reader are also in Bay 51 and access to the machines is by scheduled appointment only so the post-doc will not have to interact with other people during scheduled times. The live-cell imaging equipment is located in a Bay 52 tissue culture room and it is also accessible by scheduled appointment only. Confocal imaging and immunofluorescence microscopy can be performed at the on-campus core facility and electron microscopy can be performed at the core facility located in the Duke South Department of Pathology. Transportation between buildings can be accomplished by the Duke shuttle service or by personal vehicle for which parking passes can be made available.
Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.