Job Title: Research Assistant 3
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8935
Job Description
Position Objective

Working under limited supervision, the Research Assistant 3 will carry out complex research assignments and perform experiments on projects related to cardiac contractile function. The research assistant will work closely with other lab members and focus on research involving molecular biology of cardiac muscle contraction. This position will perform a wide array of cellular and molecular biology experiments using gel electrophoresis, protein biochemistry, western blots, bacterial transformation and culture and other in vitro techniques. The research assistant will use various molecular and computational methods to bridge the gap of understanding between structure and experimental observations in cardiac muscle physiology.

Essential Functions

Work closely with the principal investigator to carry out research projects that characterize the roles of myofilament contractile proteins in the regulation of cardiac muscle contraction. (6%) Coordinate research activities and conduct general molecular biology, including design and implement cloning strategies, PCR, mutagenesis, gel electrophoresis, gel purification, plasmid preparation, bacterial transformation, protein biochemistry, bacterial culture, western blotting and expression and purification of recombinant proteins. (25%)Devise new protocols and techniques for protein purification and protein binding studies that involve a high degree of skill and training. Carry out complex experiments to characterize binding properties of recombinant contractile proteins with various ligands using a variety of molecular and biochemical methods, including but limited to co-sedimentation assays, isothermal calorimetry, NMR, etc. (45%)Evaluate adequacy of biochemical techniques, study and test new procedures. Collect and analyze data, provide feedback on experimental design. Prepare and present research results in lab meetings. Maintain lab records. (14%) Assist in manuscript preparation and editing. May co-author research projects. (6%)

NonEssential Functions

May supervise students and technicians. (1%)Monitor laboratory budget. Oversee inventory and standard operating procedures. Maintain equipment, prepare buffer/media/reagents and dispose biohazardous waste. (2%) Perform other duties as assigned. (1%)

CONTACTS

Department: Weekly contact with investigators to discuss research, research education, direction, goals and time management. Frequent contact with laboratory employees in collaboration in common research project.

University: Occasional interaction with other faculty in the department and the university to provide updates on common collaborative research projects. Utilize core university facilities for experimentation and maintain strong relationship with core mangers for research collaborations.

External: Occasional communication with research collaborators from other institutions to exchange information.

Students: Occasional contact with students to assist with their research projects.

Supervisory Responsibility

No direct supervisory responsivities. May supervise students and technicians.

QUALIFICATIONS

Experience: 3 to 5 years of experience required. Experience with molecular biology and protein biochemistry techniques such as cell culture, protein isolation, expression and purifications preferred.

Education: Bachelor’s degree in molecular biology, biochemistry, or related scientific field required.

REQUIRED SKILLS

Knowledge of commonly used concepts, practices and procedures in chemistry, physics, biochemistry and molecular/cellular biology and in vivo cardiovascular function. Ability to perform various molecular biology techniques. Prior experience with cell culture and protein expression/purification.Experience with computer programming and molecular dynamic simulation preferred.Ability to follow instructions and pre-established guidelines to perform the functions of the job with minimal supervision.Strong organization skills and maintenance of a laboratory notebook with attention to scientific detail.Ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to effectively operate basic molecular and cellular biology laboratory equipment. Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making.Ability to work effectively, both independently and collaboratively within a team.Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues and supervisors face to face.Ability to follow through on assigned projects and provide timely follow-up as needed.Ability to meet consistent attendance.

Working Conditions

Working conditions will require working on a muscle mechanics setup, working on a bench with molecular reagents and/or cardiac tissues, working with animal models/and bacterial cells in the laboratory, working in front of a computer for several hours, working outside standard working days or hours as required. The employee will work with frequent interruptions and distractions. May have exposure to hazards. There may be exposure to research specific chemicals and the employee will need to take Chemical Safety Training. Required personal protective equipment may include a standard lab coat, gloves and safety glasses when appropriate.

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In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

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Case Western Reserve University has implemented a COVID Vaccination policy requiring evidence of COVID-19 vaccination for all students, faculty and staff with an on-campus presence. Religious and medical exemptions may be provided in accordance with applicable federal and state laws. For more information go to: https://case.edu/equity/covid-19-accommodations. Applicants may contact the Office of Equity at 216-368-3066 /equity@case.edu for information regarding religious or medical exemptions as an accommodation.

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