Research Assistant 2

Job description

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8925
Job Description POSITION OBJECTIVE

Working under general supervision, the Research Assistant 2 will plan and carry out experimental work to support researchers in projects aimed at understanding the molecular mechanisms of HIV latency including the effect of estrogen on HIV infection and latency and latency in primary TH17 cells. This position will require work with human tissue and blood samples.

ESSENTIAL FUNCTIONS

    Plan and carry out research projects in accordance with general plans. Prepare cells for use in the HIV latency models, including iPSC cells, maintain and grow lentiviral vectors and viral stocks and assist in experiments involving these materials. Research assignments include cell culture work, including coordination and pick up of blood draws; isolation of primary T-cells, viral transfections and infections; DNA isolation and manipulation (i.e., cloning, gel electrophoresis) and flow cytometry. (40%)Conduct a series of experiments including new protocols and techniques for research projects to improve the development of HIV latency models in primary T-cells and microglial cells. May assist in developing improved techniques, protocols and procedures. (12%)Collect and analyze data, prepare graphs, charts and slides from experimental data for purposes of reporting. Data analysis includes the systematic evaluation of different protocols to optimize cell viability, yield and infectivity rates. Maintain meticulous records of experiments. (14%)Train new staff, direct the work of students. Responsible for technical training of new staff, graduate students and undergraduates regarding laboratory policies as well as basic molecular virology techniques. (14%)Participate in weekly one-on-one meetings with principal investigator and weekly group laboratory meetings. Present and analyze data during weekly lab group meetings. (11%)

NONESSENTIAL FUNCTIONS

    Ensure that the laboratory complies with environmental health and safety requirements. (3%)Oversee inventory, handle purchase orders, perform general lab maintenance and tasks. (2%)Attend departmental seminars and research retreats. (2%)Perform other duties as assigned. (2%)

CONTACTS

Department: Continuous contact with supervisor, laboratory staff and faculty of the department to meet research objectives.

University: Occasional contact outside the department as needed to meet research objectives.

External: Occasional contact with vendors/businesses in regard to instrument repair and service.

Students: Frequent interactions with students within the lab and department to meet research objectives.

SUPERVISORY RESPONSIBILITY

May supervise students and technicians.

QUALIFICATIONS

Experience/Education: Bachelor’s degree in biology, chemistry, or a related field and 1 to 3 years of experience or Associate’s degree in an approved biotechnology program and 2 to 4 years of experience.

REQUIRED SKILLS

    Must possess knowledge and technical skills in: Sterile technique; reagent and media preparation; mammalian cell culture. Experience with virology and/or viral vectors preferred.Excellent communication and organizational skills, with the ability to multi-task.Ability to operate laboratory equipment including advanced instruments such as flow cytometers, liquid handling robots, PCR machines.Strong organization skills and good habit of maintaining a clean lab working environment; demonstrate attention to detail and accuracy, time management skills and proven ability to successfully follow-through on assigned projects.Must be able to and willing to learn new techniques, procedures, processes and computer software/hardware to perform experiments. Ability to work effectively independently and collaboratively within a team. Must be highly motivated, responsible, dependable and a self-starter.Professional and effective verbal and written communication skills and good interpersonal skills with the ability to work and communicate with various individuals within and external to the laboratory, Department and University.Ability to work with sensitive information and maintain confidentiality.Ability to rely on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment.Ability to meet consistent attendance.Ability to interact with colleagues, supervisors and customers face to face.Proficiency in routine computer use such as ability to use Microsoft Office and database programs.

WORKING CONDITIONS

General laboratory environment in the Department of Molecular Biology and Microbiology. Potential exposure to certain chemical and radioactive hazards. The employee will need to be able to lift up to 25 lbs., bend/stoop, crouch, push, pull and reach above shoulders. The employee will perform DOT shipping/receiving of lab items, including hazardous biological and infectious materials. Personal protective equipment (lab coat, gloves, goggles) provided to limit exposure to hazards.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

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Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.

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Job No:
Posted: 6/10/2021
Application Due: 9/8/2021
Work Type: Full Time
Salary: