Research Assistant 1

Job description

Job Title: Research Assistant 1
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8922

Working under moderate supervision, The Research Assistant 1 will perform research work in medical and related technical areas. This position will work to assist the accomplishment of Research Aims set forth in the BioSiS project. This includes the production and/or fabrication of biosensors and their components such as the biomarker recognition modules (BREs). This position will clone and heterologously express and purify proteins from E. coli and Yeast hosts, perform In vitro screening for novel Biomarker binders and assist with biophysical/biochemical characterization of BRE modules.


    Perform research work in medical and related technical areas involves use of lab skills. These consist of but are not limited to a) basic DNA manipulation including cloning via both classical restriction enzyme based approaches as well as more modern Gibson/Electra scarless methods. b) protein expression in both yeast and E. coli hosts and the purification of such from isolated cells. c) biochemical and biophysical characterization of purified proteins. This may range from protein crystallography to characterization of binding constants via inferrometry. (40%) Conduct analysis of samples. All constructs are sequenced and compared to their parent sequence as well as one another; likewise, proteins are also characterized for binding and stability. (20%) Record results for a particular experiment or closely related series of experiments. Maintain records of experiments, keep an organized and thorough laboratory notebook accessible online to all principles involved in the BioSiS project. (20%) Maintain test equipment utilized in experiments. Provide supporting details. Perform basic cleaning in the laboratory, including washing of glassware and keeping work areas clean and organized. (10%)


    Order laboratory supplies. Required reagents and supplies will be communicated to the principal investigator via email to ensure ordering in a timely manner. (5%)Perform other duties as assigned. This may include assisting in the preparation of talks, papers and annual reports. (5%)


Department: Daily contact with supervisor to discuss research and maintain workflow. This may happen both in person as well as via phone / email.

University: Infrequent contact with other departments to share information; if occurs it will happen within the BioSiS collaboration.

External: Occasional contact with the collaborative team for the BioSiS project to disseminate results. Limited or no contact with vendors to exchange information.

Students: None expected.


This position has no direct supervision of staff employees.


Education/Experience: Bachelor’s degree in science and 0 to 1 year of experience or Associate's degree in an approved biotechnology program and 0 to 2 years of experience required. Experience with Crystallographic screening, protein expression from E. coli, Saccharomyces cerevisiae and Pichia pastoris expression systems is a plus.


    Has knowledge of commonly-used concepts, practices and procedures within a particular field. Experience with basic protein purification analytical techniques preferred.Experience with DNA manipulation and cloning techniques strongly preferred.Relies on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment.Ability to meet consistent attendance.Ability to interact with colleagues and supervisors face to face.


General laboratory environment. May have exposure to hazards. Personal protective equipment, including lab coat, gloves and goggles, will be provided to limit exposure to hazards.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





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Job No:
Posted: 6/10/2021
Application Due: 6/23/2021
Work Type: Full Time