Research Specialist - EII/CRISPR
Permanent Staff (SHRA)Is this an internal only recruitment?:
Research Specialist - JourneyWorking Title:
Research Specialist - EII/CRISPRPosition Number:
P012807Budgeted Hiring Range:
$49,550 - $60,560Pay Band Information:
To learn more about the pay band for this branch and role, please see the Career Banding rates spreadsheet.Salary Grade Equivalent:
Full-Time PermanentHours per week:
SOP-Eshelman Inst for Innov-450300Date First Posted:
Chapel Hill, NCPosition Posting Category:
Research ProfessionalsDepartment Description:
The UNC Eshelman School of Pharmacy (www.pharmacy.unc.edu) is one of five health science schools (Pharmacy, Nursing, Dentistry, Public Health, Medicine) at the University of North Carolina at Chapel Hill and is one of the oldest health science academic programs at Chapel Hill. The School teaches approximately 600 PharmD students, 100 PhD students, and 20 Master’s students. The School has built a reputation for its continued pursuit of excellence, rigorous education and training programs, cutting-edge multidisciplinary research, progressive pharmacy practices, efficient business operations, and its outstanding faculty, staff, and students. The School was named the number one School of Pharmacy in the U.S. by the U.S. News and World Report. The School has experienced unprecedented growth and success and continues to strategically position itself for sustained impact, as articulated in the School’s Strategic Plan (https://pharmacy.unc.edu/about/ospa/strategic-plan/)
Our vision is to be the preeminent school of pharmacy where leaders in practice, education, and research are developed and nurtured. We are Advancing Medicine for Life through innovation and collaboration in pharmacy practice, education, research, and public service.
A new high-throughput CRISPR genetic and epigenetic screening center is being established at UNC-Chapel Hill with support from The Eshelman Institute for Innovation, Eshelman School of Pharmacy, The UNC School of Medicine, and The UNC Lineberger Comprehensive Cancer Center. This facility will house state-of-the-art screening capabilities using modern CRISPR techniques along with a highly automated screening platform.
This position will play a key role in CRISPR Screening Center. The successful candidate will apply their specialized skills culturing multiple disease-relevant cell lines and will play an important role in curation of screening libraries as well as project development for approaches to CRISPR Screening.Equal Opportunity Employer:
The University of North Carolina at Chapel Hill is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender, gender expression, gender identity, genetic information, race, national origin, religion, sex, sexual orientation, or status as a protected veteran.Position Description:
This position will play a key role in CRISPR Screening Center. The successful candidate will apply their specialized skills culturing multiple disease-relevant cell lines and will play an important role in the curation of screening libraries as well as project development for approaches to CRISPR Screening. This position is responsible for:
5% Interacting and coordinating with users to adapt TC conditions to high-throughput format.
15% Curation, amplification, and QC of a comprehensive library of CRISPR expression vectors and commercial pooled libraries.
20% Design, synthesis, and QC of custom, user-defined pooled CRISPR libraries:Transform library into bacteria for amplification. Transformation is the process by which foreign DNA is introduced into a cell. The transformation of bacteria with plasmids is important because bacteria are used as the means for replicating plasmids.Amplify plasmid library. Amplification is necessary to produce sufficient quantities of the library for experimental screening.Verify (QC) library completeness with next-generation sequencing.
15% Production and titer of lentivirus for pooled CRISPR screens:
Determine the sensitivity of target cell type to puromycin (kill curve). Determine CFU/ml (colony forming units per ml) for the target cell type using a control lentivirus sample similar in nature to the pooled library. Seed and transduce cells. Treat and cell harvest. Master mix preparation. Run PCR and calculate the titers.
15% Adaptation of pooled and high-throughput screens for desired experimental readouts:
Pooled screens generate relatively clear-cut readouts, such as cell proliferation, cell death, or sortable marker proteins, and uses next-gen sequencing.
For more complex phenotypic readouts, arrayed or high-throughput screens are developed incorporating high content imaging.
15% Design, optimization, and dissemination of SOP workflows for validation of Cas9/dCas9 function and target knockout, knockdown, and overexpression
10% Properly preparing samples for and performing next-generation sequencing for pooled library screens.
5% Other duties as assignedMinimum Education and Experience Requirements:
Bachelor’s degree in a discipline related to the area of assignment; or equivalent combination of training and experience. All degrees must be received from appropriately accredited institutions.Essential Skills, Knowledge and Abilities:
Bachelor’s degree in a discipline related to the area of assignment; or equivalent combination of training and experience. All degrees must be received from appropriately accredited institutions.Preferred Qualifications:
Specialized skills in culturing multiple disease-relevant cell lines and in the curation of screening libraries are preferred. Experience in project development for approaches to CRISPR Screening is preferred.Stimulus/ARRA Funded: