Professional Research Assistant
University of Colorado Denver School of Medicine
The University of Colorado Denver l Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment. We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.JOB SUMMARYThis PRA position is a full-time, 1.0 FTE research position to work on virology, molecular biology, immunology, and pathogenesis of infection and cancer. This full-time position involves research in virology, immunology, molecular biology and pathogenesis, including work to analyze recombinant viruses, cell culture systems and mouse models. Responsibilities include work with cell and molecular biology, virus growth and quantitation, recombinant DNA work, multiparametric flow cytometry, analysis of viruses and host responses in mouse models for determination of virus infection and of disease, including tumorigenesis. The position requires carefully following instructions for a wide variety of laboratory procedures that will be demonstrated or described, keeping excellent, well organized and up-to-date records of experiments, organizing samples for later analyses, ordering supplies in a timely and efficient manner, maintaining equipment in proper working order or promptly arranging for repair of instruments. Maintaining a neat and orderly laboratory is required. Laboratory administrative duties include placing orders for lab supplies, mailing packages to colleagues, helping with preparation of grant applications and manuscripts, preparation of slides and figures, and maintaining financial records. Occasionally, the incumbent will prepare and present results for presentation and group discussions and will instruct others in laboratory techniques when requested by the lab head. The incumbent is expected to work effectively with students, post-doctoral fellows and other lab members.SUPERVISION RECEIVEDThe employee will be supervised by the heads of the laboratory, Dr. Linda van Dyk and Dr. Eric Clambey, and will work with members of laboratory staff as assigned.SUPERVISION EXERCISEDAfter initial training in animal work, cell culture and other techniques, this PRA may also help to train and supervise the undergraduate student workers and rotating graduate students or medical students.EXAMPLES OF WORK PERFORMED
- Direct involvement in murine studies, including virus pathogenesis, lung inflammation (including tumorigenesis), followed by tissue isolation, processing and analysis. These experiments often involve time courses from days to weeks.
- Multi-parameter flow cytometric analysis.
- Viral propagation and quantitation using cell culture and plaque assays.
- Harvest and asceptic purification of primary leukocyte populations followed by in vitro culture and manipulation.
- Tissue culture work including feeding and splitting of cultured cell lines, and work with transient and stable transfection methodologies, including plasmid-based luciferase assays and siRNA silenced cell lines.
- Molecular biology work including DNA and RNA purification, polymerase chain reaction (PCR) and real-time reverse transcriptional polymerase chain reaction (RT-PCR), protein analysis, including antibody-based flow-cytometric analysis and enzyme-linked immunosorbent assay (ELISA); histological techniques such as tissue sections, staining and immunohistochemistry.
- All research tasks require careful data gathering and documentation.
- Assistance with data interpretation and analysis.
- Lab ordering, record keeping and maintenance.
- B.S. or B.A. degree in microbiology, molecular biology, biochemistry, genetics, cell biology, immunology or closely related field is required.
- Previous laboratory experience in molecular biology, cell culture, virology, flow cytometry, handling of laboratory animals and computer/administrative skills. Experience in immunology and RNA biology is desirable, as is knowledge of R or Python.
- Good computer skills are required. Applicants should demonstrate they are well organized, reliable, independent, and have excellent verbal and written communication skills. Applicants should be able to quickly adapt to new challenges, show willingness to learn and have good interaction skills.
Job Category: Faculty
Primary Location: Aurora
Posting Date: Jun 7, 2021
Unposting Date: Ongoing