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Research Assistant 2

Case Western Reserve University
Case Main Campus
Closing date
Jun 28, 2021

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Job Details

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8898

Working under general supervision, the Research Assistant 2 will plan and carry out research projects in accordance with general plans focused on understanding the pathogeneses and improving the early diagnoses of neurodegenerative diseases including prion diseases, Parkinson’s disease and Alzheimer’s disease. This individual will perform research experiments based on specific instructions/protocols including one and two-dimensional Western blotting, immunohistochemistry, cell culture, animal models, aggregation-seeding activity, and microscopy with little aid by supervising staff. Data will be collected and recorded in lab computers for statistical analyses and this individual will contribute to the generation of data figures for grant submissions and publication of manuscripts, as well as present data at group meetings. This individual will proactively follow their job duties while adhering to and ensuring ethical compliance with all legal regulations. They will also ensure strict adherence to all safety guidelines while dealing with hazardous substances, animal subject- and human subject-derived materials in the lab. The research assistant will be involved in animal work, which involves monitoring movement and behavior disorders in mouse models with prion diseases, Parkinson’s disease, or Alzheimer’s disease twice a week. The research assistant will euthanize mice carbon dioxide gas and sacrifice mice at different time points to collect brain and skin samples for RT-QuIC and PMCA assays.


    Plan and carry out research assignments in accordance with lab plans set up by the principal investigator. Experiments may include Western blotting, immunohistochemistry, real-time quaking-induced conversion assay, protein misfolding cyclic amplification assay, ELISA, tissue culture, common molecular biology techniques and in vivo studies using animals. (60%)Conduct a series of experiments to supply scientific information as part of a larger project. Studies include but are not limited to analysis of seeding activity of misfolded proteins with RT-QuIC and sPMCA in animal and human autopsy samples, culture of cell lines, Western blotting, ELISA, DNA and RNA isolation, PCR, immunofluorescence, transfections, generation of retroviral and lentiviral vectors as well as transduction of mammalian cells using these vectors, gene silencing, immunoblots and immunoprecipitation. (15%)Assist in developing improved techniques, projection methods or procedures in selection of cell lines, authentication, metabolic aspects on pancreatic cancer to entail optimization of established protocols. (10%)


    Collect and analyze data. Provide data in a presentable format for use by the principal investigator and other lab members as they generate annual reports for granting agencies, manuscripts, and grant and pilot applications. Manage individual experiments, reporting these in a consistent fashion and in a timely manner, managing issues of the scientific method including positive and negative controls. (5%)Oversee inventory of supplies necessary for daily laboratory work. Order reagents and other laboratory supplies. Perform general laboratory support tasks including stocking communal reagents and supplies, organizing lab spaces, and receiving deliveries. Install, maintain, and repair complex equipment. (~4%)Train new staff regarding standard laboratory policies as well as basic biochemistry research techniques. (5%)Perform other duties as assigned. (<1%)


Department: Daily contact with supervisor to discuss research and maintain workflow

University: Occasional contact with other departments to share information and help enable collaborations. Limited interaction with regulatory components (IACUC, IBC, IRB)

External: Occasional contact with vendors to procure supplies

Students: Occasional contact with student employees to explain policies and procedures and ensure proper adherence to all legal, health and safety standards


This position has no direct supervision of staff employees but may train new staff and direct the work of students.


Experience: Bachelor’s degree in science and 1 to 3 years of related experience or Associate’s degree in an approved biotechnology program and 2 to 4 years of experience.


    Has knowledge of commonly-used concepts, practices, and procedures within pathology. Relies on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment.Ability to meet consistent attendance.Ability to interact with colleagues, supervisors and customers face to face.Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center’s mission. Must always handle animals with care and respect. Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals. Previous experience working with animals preferred. Strong molecular biology skills (PCR, Western blots, Retroviral/Lentiviral constructs).Strong organizational skills and good habit of maintain a clean lab working environment; demonstrate attention to detail and accuracy, time management skills, and proven ability to successfully follow-through on assigned projects.Professional and effective verbal and written communication skills and good interpersonal skills with the ability to work and communicate with various individuals within and external to the university.Ability to work effectively independently and collaboratively within a team (must be highly motivated, responsible, dependable, and a self-starter). Ability to work with sensitive information and maintain confidentiality.Proficiency in Microsoft Office and GraphPad Prism is preferred. Experience using Adobe Photoshop, and Illustrator.Must have the ability to maintain meticulous, complete, and easily retrievable laboratory data.Must have the ability to willingly learn new techniques and procedures as needed, follow established protocols or laboratory procedures, and request clarification if necessary.


General laboratory environment. May have exposure to chemicals., bloodborne pathogens, repetitive motion, fumes, dust, pesticides or herbicides, animals, and select agents. The employee will be provided with protective eyewear, lab coat, and appropriate gloves. The employee will need to be able to balance, bend/stoop, climb stairs, crawl, crouch, climb ladders, kneel, keyboard/ type, push/ pull, reach, reach above shoulder, perform repetitive motion, squat, and use computer mouse.

Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the “Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals.” This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.



Working at Case Western Reserve University

Case Western Reserve University is among the nation's leading research institutions. Founded in 1826 and shaped by the unique merger of the Case Institute of Technology and Western Reserve University, Case Western Reserve is distinguished by its strengths in educationresearch, service and experiential learning. Located in Cleveland's University Circle, we offer nationally recognized programs in the arts and sciencesdental medicineengineeringlawmanagementmedicinenursing and social sciences.

Student enrollment exceeds 9,800 students, forty percent of whom are undergraduatesFacultyand students hail from more than 90 countries, with academic interests that reach every region of the world. Case Western Reserve, with the support of individuals, corporations and foundations, aids nearly 100 designated research centers.

As a service-oriented institution dedicated to civic leadership, Case Western Reserve seeks individually and collectively to prepare our students to improve the human condition and to direct the benefits of discovery toward a better society. This effort is not limited to the university's classrooms, laboratories, librariesresidence halls and athletic fields, but includes partnerships with many other institutions. We build these partnerships believing that our ability to improve the human condition should begin in our own community.

Case Western Reserve University remains Ohio's top-ranked school among the nation's premier national universities, as ranked by U.S. News & World Report and is the only Ohio institution ranked in the top 50 nationally. We promote a culture of inquiry marked by rigor, creativity, curiosity, innovation, respect, sensitivity and open communication of ideas.

The university supports interdisciplinary partnerships in education and research with numerous faculty holding joint appointments in more than one Case Western Reserve school or department, as well as at neighboring institutions. These relationships launched many of the unique pairings of science, business and liberal arts into the centers and programs that enhance the undergraduate and graduate experience at the university. Our dual degree programs enrich the educational experience for those students who choose to balance the technical requirements of engineering or the sciences with a strong interest in the humanities.

With more than $375 million in research funding annually, the university attracts outstanding undergraduate and graduate students along with renowned faculty whose research has made significant contributions to the way we live and work. Case Western Reserve counts 15 Nobel laureates among our alumni and current and former faculty, including the first American scientist to ever receive the prize.

Our students' experiences in cultural institutions, clinics, social service agencies and industry are not viewed as “extra-curricular,” but create the learning experience that defines a Case Western Reserve education. The university's service programs in the Cleveland Municipal School District have impacted more than 145,000 students with more than 43 signature programs. The university has launched more than 580 community partnerships on the local, national and international levels. Annually the university sponsors Case for Community Day, an event where hundreds of Case Western Reserve employees and students lend their time and talent to targeted community development projects in the Greater Cleveland area.

Our alumni number more than 110,000 and constitute a “who's who” of every profession. Alumni represent one of the university's strongest resources, playing a vital role in campus life through their work as advisors, mentors, friends and advocates.

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