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Research Assistant 2

Case Western Reserve University
Case Main Campus
Closing date
Jun 28, 2021

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Academic Affairs, Research Staff & Technicians
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Job Details

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8893

Working under general supervision, the Research Assistant 2 will plan and carry out research projects in the laboratory focusing on: (1) the movement of carbon dioxide (CO2), oxygen (O2), nitrogen (N2) through the plasma membrane, including through protein channels in the membrane; and (2) the regulation of intracellular pH (pHi) by membrane transport proteins, including the broader field of acid-base homeostasis; and (3) membrane-protein sensors for extracellular CO2 and HCO3. The research assistant will be primarily responsible for conducting experiments to record surface and intracellular pH and intracellular ion concentrations in assays that measure gas, HCO3, CO3= and water fluxes. The research assistant will also fabricate equipment and solutions, support laboratory staff and assist with laboratory maintenance. This position will work with animals.


    Plan and carry out experiments under principal investigator supervision to quantify the transmembrane flux CO2, HCO3, CO3=, NH3, NH4+ and H2O via aquaporins, Rhesus proteins, Na-coupled bicarbonate transporters and other candidate gas channel proteins when expressed in Xenopus laevis oocytes. This includes performing the surgeries to harvest the oocytes from the frogs. (50%)Conduct standard molecular biology and biochemistry protocols to clone, mutate and quantify expression of our genes of interest in heterologous expression systems. (15%)Collect and maintain accurate and thorough records of experimental and other research data. (15%)Perform statistical analyses to compare the results of the different experimental conditions. Generate figures and reports summarizing the experimental findings. May assist in developing improved techniques, projection methods or procedures. (10%)


    Oversee inventory. May make design modifications to laboratory equipment. Train new staff; direct the work of students. (5%) Perform other duties as assigned. (5%)


Department: Daily contact with the principal investigator, instructors, postdocs, research associates and students within the research group to discuss research.

University: Regular contact with principal investigators or laboratory personnel in other research groups or core facilities to discuss research. Occasional contact with personnel in other departments when using shared laboratory to exchange expertise or information.

External: Limited contact with vendors to exchange information.

Students: Daily contact with students in the laboratory to instruct them in the use of equipment and/or make adjustments to procedures.


This position has no direct supervision of staff employees. Train new staff and direct the work of students.


Experience/Education: Bachelor's degree and 1 to 3 years of experience or Associate’s degree in an approved biotechnology program and 2 to 4 years of experience required. Strong background in biology, chemistry, physics and math preferred. Experience in transport physiology, animal physiology, or experimental biology is preferred.


    Outstanding fine motor skills and manual dexterity.Ability to maintain research focus on a technically challenging project.Previous experience working with animals preferred.Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Strong organization skills and attention to scientific detail. Ability to maintain a laboratory notebook.Ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to effectively operate laboratory equipment.Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making.Ability to work effectively, both independently and collaboratively within a team.Technical competence with software programs such as Microsoft Office (Word, Excel and PowerPoint) and scientific graphics package (such as Origin) is strongly preferred. Ability to learn and apply software programs and applications as needed.Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.Excellent verbal and written communication skills; good interpersonal skills with the ability to interact effectively with colleagues, supervisors and customers face to face.Ability to meet consistent attendance.


The employee will be working in a laboratory environment with general chemicals and will need to take Chemical Safety Training. Required personal protective equipment includes a standard lab coat, gloves and safety glasses when appropriate.

Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.



Working at Case Western Reserve University

Case Western Reserve University is among the nation's leading research institutions. Founded in 1826 and shaped by the unique merger of the Case Institute of Technology and Western Reserve University, Case Western Reserve is distinguished by its strengths in educationresearch, service and experiential learning. Located in Cleveland's University Circle, we offer nationally recognized programs in the arts and sciencesdental medicineengineeringlawmanagementmedicinenursing and social sciences.

Student enrollment exceeds 9,800 students, forty percent of whom are undergraduatesFacultyand students hail from more than 90 countries, with academic interests that reach every region of the world. Case Western Reserve, with the support of individuals, corporations and foundations, aids nearly 100 designated research centers.

As a service-oriented institution dedicated to civic leadership, Case Western Reserve seeks individually and collectively to prepare our students to improve the human condition and to direct the benefits of discovery toward a better society. This effort is not limited to the university's classrooms, laboratories, librariesresidence halls and athletic fields, but includes partnerships with many other institutions. We build these partnerships believing that our ability to improve the human condition should begin in our own community.

Case Western Reserve University remains Ohio's top-ranked school among the nation's premier national universities, as ranked by U.S. News & World Report and is the only Ohio institution ranked in the top 50 nationally. We promote a culture of inquiry marked by rigor, creativity, curiosity, innovation, respect, sensitivity and open communication of ideas.

The university supports interdisciplinary partnerships in education and research with numerous faculty holding joint appointments in more than one Case Western Reserve school or department, as well as at neighboring institutions. These relationships launched many of the unique pairings of science, business and liberal arts into the centers and programs that enhance the undergraduate and graduate experience at the university. Our dual degree programs enrich the educational experience for those students who choose to balance the technical requirements of engineering or the sciences with a strong interest in the humanities.

With more than $375 million in research funding annually, the university attracts outstanding undergraduate and graduate students along with renowned faculty whose research has made significant contributions to the way we live and work. Case Western Reserve counts 15 Nobel laureates among our alumni and current and former faculty, including the first American scientist to ever receive the prize.

Our students' experiences in cultural institutions, clinics, social service agencies and industry are not viewed as “extra-curricular,” but create the learning experience that defines a Case Western Reserve education. The university's service programs in the Cleveland Municipal School District have impacted more than 145,000 students with more than 43 signature programs. The university has launched more than 580 community partnerships on the local, national and international levels. Annually the university sponsors Case for Community Day, an event where hundreds of Case Western Reserve employees and students lend their time and talent to targeted community development projects in the Greater Cleveland area.

Our alumni number more than 110,000 and constitute a “who's who” of every profession. Alumni represent one of the university's strongest resources, playing a vital role in campus life through their work as advisors, mentors, friends and advocates.

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