Research Assistant 2 Location:
Case Main Campus Regular/Temporary:
Regular Full/Part Time:
Full-Time Job ID:
Job Description POSITION OBJECTIVEWorking under general supervision, the Research Assistant 2 will plan and carry out research projects in the laboratory focusing on: (1) the movement of carbon dioxide (CO2
), oxygen (O2
), nitrogen (N2
) through the plasma membrane, including through protein channels in the membrane; and (2) the regulation of intracellular pH (pHi
) by membrane transport proteins, including the broader field of acid-base homeostasis; and (3) membrane-protein sensors for extracellular CO2
. The research assistant will be primarily responsible for conducting experiments to record surface and intracellular pH and intracellular ion concentrations in assays that measure gas, HCO3−
and water fluxes. The research assistant will also fabricate equipment and solutions, support laboratory staff and assist with laboratory maintenance. This position will work with animals. ESSENTIAL FUNCTIONS.
Plan and carry out experiments under principal investigator supervision to quantify the transmembrane flux CO2, HCO3−, CO3=, NH3, NH4+ and H2O via aquaporins, Rhesus proteins, Na-coupled bicarbonate transporters and other candidate gas channel proteins when expressed in Xenopus laevis oocytes. This includes performing the surgeries to harvest the oocytes from the frogs. (50%)Conduct standard molecular biology and biochemistry protocols to clone, mutate and quantify expression of our genes of interest in heterologous expression systems. (15%)Collect and maintain accurate and thorough records of experimental and other research data. (15%)Perform statistical analyses to compare the results of the different experimental conditions. Generate figures and reports summarizing the experimental findings. May assist in developing improved techniques, projection methods or procedures. (10%)
Oversee inventory. May make design modifications to laboratory equipment. Train new staff; direct the work of students. (5%) Perform other duties as assigned. (5%)
CONTACTS Department: Daily contact with the principal investigator, instructors, postdocs, research associates and students within the research group to discuss research.University: Regular contact with principal investigators or laboratory personnel in other research groups or core facilities to discuss research. Occasional contact with personnel in other departments when using shared laboratory to exchange expertise or information.External: Limited contact with vendors to exchange information.Students: Daily contact with students in the laboratory to instruct them in the use of equipment and/or make adjustments to procedures. SUPERVISORY RESPONSIBILITY This position has no direct supervision of staff employees. Train new staff and direct the work of students. QUALIFICATIONSExperience/Education: Bachelor's degree and 1 to 3 years of experience or Associate’s degree in an approved biotechnology program and 2 to 4 years of experience required. Strong background in biology, chemistry, physics and math preferred. Experience in transport physiology, animal physiology, or experimental biology is preferred. REQUIRED SKILLS
Outstanding fine motor skills and manual dexterity.Ability to maintain research focus on a technically challenging project.Previous experience working with animals preferred.Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Strong organization skills and attention to scientific detail. Ability to maintain a laboratory notebook.Ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to effectively operate laboratory equipment.Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making.Ability to work effectively, both independently and collaboratively within a team.Technical competence with software programs such as Microsoft Office (Word, Excel and PowerPoint) and scientific graphics package (such as Origin) is strongly preferred. Ability to learn and apply software programs and applications as needed.Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.Excellent verbal and written communication skills; good interpersonal skills with the ability to interact effectively with colleagues, supervisors and customers face to face.Ability to meet consistent attendance.
WORKING CONDITIONSThe employee will be working in a laboratory environment with general chemicals and will need to take Chemical Safety Training. Required personal protective equipment includes a standard lab coat, gloves and safety glasses when appropriate.Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines. Diversity Statement
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.