Research Assistant 1 Location:
Case Main Campus Regular/Temporary:
Regular Full/Part Time:
Full-Time Job ID:
Job Description POSITION OBJECTIVE
Working under moderate supervision, the Research Assistant 1 will perform research work and support scientists by performing standard molecular techniques (RNA extraction, qPCR, protein analysis by Western blotting, immunofluorescence, or immunohistochemistry), culturing cells and assessing tumor growth in animals. The research assistant will also collect, verify, record, and compile data and information related to the ongoing research carried out in the lab. The research assistant will be responsible for general laboratory maintenance. This position will work with animals.
Perform research work in medical and related technical areas involves use of lab skills. Perform research on human and murine cells treated with cancer therapies, biological substances (growth factors, cytokines, chemokines), or viruses to genetically manipulate the cells in vitro (30%). Perform research using animals in vivo to model cancer development and therapy. Perform research on human and murine cells transplanted into animals that will be treated with cancer therapies and biological substances (growth factors, cytokines, chemokines) to test response to therapy (10%). Conduct analysis of samples collected from in vitro and in vivo studies, including common molecular biology techniques to assess how cells and tissues respond to treatment or genetic manipulation. Techniques commonly used include RNA extraction and qPCR, protein analysis by Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry (22%).Record results of experiments. Collect and record cell growth or tumor growth over time by counting, measuring, and using bioluminescence procedures. Quantify target protein or gene expression using statistical methods. Maintain laboratory manuals. (17%). Order laboratory supplies. Place orders through university channels or through direct contact with vendors (6%).Maintain test equipment utilized in experiments. Make reagents stocks and solutions for lab use. Organize lab reagents (plasmids and reagents in freezers/refrigerators). Test cell and organize cell lines in liquid nitrogen stocks (6%). Manage research waste through university protocols (6%).
NONESSENTIAL FUNCTIONS Perform other duties as assigned. (3%) CONTACTS Department: Continuous contact with supervisor and lab members to discuss research and maintain workflow and order supplies.University: Infrequent contact with other departments to share information and collaborate on projects. Interaction with regulatory components (EHS, IACUC, IBC, IRB).External: Infrequent contact with vendors to procure supplies.Students: Continuous contact with graduate students within the laboratory to provide technical assistance, to gather information for ordering of supplies, and to update policies and procedures manuals. SUPERVISORY RESPONSIBILITY This position has no direct supervision of staff employees. QUALIFICATIONSEducation/Experience: Bachelor’s degree in science and 0 to 1 year of experience or Associate's degree in an approved biotechnology program and 0 to 2 years of experience required. REQUIRED SKILLS
Has knowledge of commonly-used concepts, practices, and procedures within the field of molecular and cellular biology; experience with the field of cancer research preferred. Relies on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment (electrophoresis equipment, PCR machine, centrifuges, microscopes, tissue culture hood).Ability to meet consistent attendance.Ability to interact with colleagues and supervisors face-to-face.Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Strong organization skills and good habit of maintaining a clean lab working environment; demonstrate attention to detail and accuracy, time management skillsProfessional and effective verbal and written communication skills and good interpersonal skills with the ability to work and communicate with various individuals.Must be highly motivated, responsible, dependable and a self-starter. Must have the ability to maintain meticulous, complete, and easily retrievable laboratory data.Must have the ability to willingly learn new techniques and procedures as needed, follow established protocols or laboratory procedures and request clarification if necessary.
WORKING CONDITIONSGeneral laboratory environment. The employee will be exposed to blood-borne pathogens and chemicals. Employee will need to wear appropriate protective equipment such as gloves, coat, and eyewear. Working conditions will require working at the bench in a molecular genetics/ biology laboratory, working with mice and other animal models in the laboratory and in the animal facility.Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines. Diversity Statement
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.