DNA Sequencing Technician (9613C) 18177 About Berkeley

At the University of California, Berkeley, we are committed to creating a community that fosters equity of experience and opportunity, and ensures that students, faculty, and staff of all backgrounds feel safe, welcome and included. Our culture of openness, freedom and belonging make it a special place for students, faculty and staff.

The University of California, Berkeley, is one of the world's leading institutions of higher education, distinguished by its combination of internationally recognized academic and research excellence; the transformative opportunity it provides to a large and diverse student body; its public mission and commitment to equity and social justice; and its roots in the California experience, animated by such values as innovation, questioning the status quo, and respect for the environment and nature. Since its founding in 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world.

We are looking for equity-minded applicants who represent the full diversity of California and who demonstrate a sensitivity to and understanding of the diverse academic, socioeconomic, cultural, disability, gender identity, sexual orientation, and ethnic backgrounds present in our community. When you join the team at Berkeley, you can expect to be part of an inclusive, innovative and equity-focused community that approaches higher education as a matter of social justice that requires broad collaboration among faculty, staff, students and community partners. In deciding whether to apply for a position at Berkeley, you are strongly encouraged to consider whether your values align with our Guiding Values and Principles, our Principles of Community, and our Strategic Plan.

Application Review Date

The First Review Date for this job is: May 20, 2021

Departmental Overview

The UC Berkeley DNA Sequencing Facility is one of the scientific recharge facilities managed by the Molecular and Cell Biology Department. The facility supports research by providing Sanger sequencing, Genotyping, High-Throughput Nucleic Acid Purification/Quantification, PCR Reaction Cleanup, PCR products Size Selection, Human Cell Line Authentication, Open Access Instruments and free consultation services as requested by members of roughly 200 research labs on and off campus recharging $1,000,000 yearly. Approximately 20,000~30,000 DNA sequence requests are processed each month.

This role will work with the Director, the assistant Director, staff research associates and under graduate laboratory assistant in 10AM-6PM shifts and assists in day to day operation and maintenance of the DNA sequencing facility.

Responsibilities

• Perform DNA sequencing data analysis using ABI Sequence Analysis Software and e-mail sequence results to customers. Maintain precise records of all work. Help collect DNA samples from five drop off locations on campus, prepare DNA sequencing samples by organizing and setting up reactions for cycle sequencing reactions. Enter sample and customer names to DNA database and schedule sequencing runs on ABI 3730 XL DNA Analyzers. Perform clean up of DNA sequencing reactions with CleanSEQ Dye Terminator Clean-Up system using Beckman Coulter Biomek NX Automated Liquid Handlers. Prepare buffers and solutions. Set up DNA Analyzers and Biomek NX workstations ready for the samples. Perform high-throughput plasmid purification and PCR cleanup reactions using Beckman Biomek Automated liquid handlers as needed. Use a spectrophotometer to evaluate DNA quality and quantity and prepare samples from those procedures for sequencing. Set up genotyping samples by creating run modules according to the fragment size and the dye-sets used and change the filter set in ABI 3730XL for the particular dye-set. Evaluate genotyping run results and email to customer.
• Perform weekly re-start of four 3730XL DNA Analyzers, three Beckman Coulter Biomek NX robots and the computers. Clean three ABI 3730XL DNA Analyzers and load new polymers regularly. Change capillary arrays and run matrix standard when necessary. Perform instrument calibrations and regular maintenance work on three DNA Analyzers and two Biomek NX automated liquid handlers (monthly: spatial calibrations. Weekly: equipment re-start, preventative maintenance for memory issues, framing of Biomek NXs) and record data on computers. Troubleshoot equipment problems and make adjustments to improve performance or set up a service call to solve the problem. Act as liaison with Applied Biosystems, Beckman Coulter or other Companies for equipment or troubleshooting problems.
• Help training of the new hires and supervision of student assistants. Assist researchers with setting up their new genotyping and sequencing projects and troubleshoot the failed or poor results. Advise researchers with their experimental designs and technical aspects of DNA preparation, primer design, etc. Keep up to date with technological advances in DNA sequencing technologies and attend workshops, seminars and meetings. Help with researching and developing new sequencing protocols for handling difficult templates, testing protocols and making recommendations to manager. Assist in development and maintenance of standard operating procedures documentation for general lab reference.

Required Qualifications

• Bachelor's degree in Molecular Biology, Biochemistry, Chemistry or related field plus hands on research experience and/or equivalent experience training.
• Must be a team player and adaptable to changes in work environment.
• Experience in medium to high-throughput environment is essential.
• Strong computer skills and extensive experience and knowledge of DNA sequencing and genotyping.
• Advanced knowledge of a wide variety of specialized laboratory equipment, including the automated sequencers, thermal cyclers, and automated liquid handlers
• Experience in programming Biomek Control Software (Beckman-Coulter), operating, maintaining, troubleshooting, and calibration of automated liquid handlers.
• Knowledge of necessary lab safety procedures and requirements, as well as chemical disposal guidelines.
• Ability to work independently in an efficient manner and with attention to details
• Excellent interpersonal, organizational and oral/written communication skills are must.

Preferred Qualifications

• Advanced knowledge and expertise associated with nucleic acid enzymology and cloning techniques including polymerases, nucleases, and restriction enzymes and cloning/expression vectors.
• Excellent computer skills including networking, Python, and R.

Salary & Benefits

This is an hourly paid position (stepped rates).

For information on the comprehensive benefits package offered by the University visit:

https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html

How to Apply

Please submit your cover letter and resume as a single attachment when applying.

Other Information

This is a part-time (20 hours per week) career position that is eligible for full benefits.

This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at: http://ucnet.universityofcalifornia.edu/labor/bargaining-units/rx/index.html

Conviction History Background

This is a designated position requiring fingerprinting and a background check due to the nature of the job responsibilities. Berkeley does hire people with conviction histories and reviews information received in the context of the job responsibilities. The University reserves the right to make employment contingent upon successful completion of the background check.

Equal Employment Opportunity

The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see:

https://www.eeoc.gov/sites/default/files/migrated_files/employers/poster_screen_reader_optimized.pdf
For the complete University of California nondiscrimination and affirmative action policy see:
http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct



To apply, visit https://careerspub.universityofcalifornia.edu/psp/ucb/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=21&JobOpeningId=18177&PostingSeq=1





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