University of Colorado School of Medicine
University of Colorado | CU Anschutz Medical Campus
School of Medicine, Department of Pediatrics
Section of Hematology, Oncology, and Bone Marrow Transplantataion Research
Position #00613155– Requisition #21010
* Applications are accepted electronically ONLY at www.cu.edu/cu-careers *The University of Colorado Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment. We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.The Department of Pediatrics, Section of Hematology, Oncology, and Bone Marrow Transplantataion Research has an opening for a full-time Research Associate position. Nature of Work:
- This position is part of an exciting research program to understand the molecular mechanisms of brain tumor biology. The laboratory uses cellular and mouse models to recapitulate the human disease, with an emphasis on epigenetics in medulloblastoma. We are looking for a PhD scientist who is interested in studying the role of histone modifications in brain tumors using standard molecular biology techniques and genome wide analysis such as ChIP-Seq, and array based expression profiling or RNA-Seq. We are also looking for experience with in vivo models of brain development/disease/tumors. Knowledge of epigenetic regulation and mechanism of gene expression in stem cell specification and differentiation is highly desired.
- Cell culture of tumor cells. 15%
- Transfection of histone modifying enzymes into cells. 15%
- Measuring consequences of Histone modifications by western blotting, qPCR, Chip-Seq and gene expression profiling. 15%
- Evaluating phenotypic consequences of histone modifications by measuring cell proliferation, cell self-renewal, cell death and senescence using standard cell biology techniques such as flow cytometry, cell culture, and
- immunohistochemistry. 15%
- Creating tumors in mice and evaluating the consequences of alternations in histone modifications on tumor development and growth. 10%
- Set up mouse breeding/genotypying and maintain mouse colonies. 10%
- Independently performed lab experiments. 5%
- Collection and analysis of all data. 5%
- Synthesis of data presenting data at lab meetings, reviewing literature relevant to project and writing of manuscript. 5%
- Maintain accurate and current laboratory notebook including all experimental details. 5%
- Graduation from an accredited university with a PhD in Genetics, Cancer Biology, Biochemistry, Molecular Biology, or related field.
- Minimum of 3 years of Cancer/Molecular Biology lab experience.
- Must be willing and able to pass a national criminal background check.
- Prior experience with murine cancer or brain disease models.
- Experience with stem cell biology and relevant assays (flow cytometric analysis and flow sorting of stem or progenitor population.
- Prior experience in chromatin biology.
- Ability to function autonomously but exercise sound judgment in seeking appropriate supervision.
- Skilled in communicating effectively and facilitating group processes.
- Ability to establish and maintain effective working relationships with sponsors, researchers, and other team members.
- Comply with all standard operating procedures and quality control systems per the University of Colorado policy.
- Comply with any laboratory duties assigned by the PI.
- Knowledgeable of epigenetic regulation and mechanism of gene expression in stem cell specification.
- Demonstrates the proficiency in computer software, equipment operation, or research technical skills.
- Excellent communication skills in English.
Job Category: Faculty
Primary Location: Aurora
Posting Date: Apr 28, 2021
Unposting Date: May 4, 2021, 5:59:00 AM