Research Assistant 2

Job description

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8789

Working under general supervision, the Research Assistant 2 will plan and carry out research projects in accordance with general research guidelines. The research assistant will work closely with other lab members and be responsible for analyzing data and presenting research results in scientific meetings. This position will focus on research involving molecular mechanisms of cardiac muscle contraction in models of heart failure, will be expected to assist in performing experiments of in vivo function in transgenic mouse models and will perform a wide array of cellular and molecular experiments using native cardiac tissues and recombinant proteins. Duties include planning and performing experiments on projects related to cardiac contractile function. This position will work with animals.


    Plan and carry out research projects that characterize the roles of myofilament contractile proteins in the regulation of cardiac muscle contraction. (25%) Manage animal resources used in experiments by keeping detailed records of animal inventory, setting up and checking breeding pairs for litters and separating cages at time of weaning. (10%)Conduct in vivo gene therapy experiments using intravenous and retro-orbital injection of AAV9 vectors in mice. (15%)Conduct a wide range of biochemical and functional studies of cardiac mechanics including: gel electrophoresis, gel purification, western blotting, kinase experiments, immunohistochemistry, expression and purification of recombinant proteins, and small animal echocardiography. (20%)Collect, organize and analyze research data for presentation in laboratory meetings. (15%)May assist in developing more efficient gene therapy and biochemical techniques. (5%)


    Oversee inventory and standard operating procedures. Maintain equipment, prepare buffer/media/reagents, and dispose biohazardous waste. Maintain lab records. Train new staff; direct the work of students. (5%)Perform other duties as assigned. (5%)


Department: Daily contact with faculty and investigators to discuss research, research education, direction, goals and time management. Frequent contact with laboratory employees to collaborate on common research projects.

University: Occasional contact with other faculty in the university to provide updates on common collaborative research projects. Occasional contact with core university facilities for experimentation and maintenance of strong relationships with core managers for research collaborations.

External: Limited contact with vendors to exchange information.

Students: Occasional contact with students to assist with research projects.


No direct supervisory responsibilities. Train new staff and direct the work of students.


Education and Experience: Bachelor’s degree in Molecular Biology or related field and 1 to 3 years of related experience or Associate’s degree in an approved biotechnology program and 2 to 4 years of related experience required.


    Knowledge of commonly used concepts, practices and procedures in chemistry, physics, biochemistry and molecular/cellular biology and in vivo cardiovascular function. Prior experience with cell culture, protein expression/purification, confocal microscopy, small animal surgery, muscle mechanics experiments. Previous experience working with animals preferred. Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals.Ability to follow instructions and pre-established guidelines to perform the functions of the job with minimal supervision.Strong organization skills and maintenance of laboratory records, with attention to scientific detail.Ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to effectively operate basic molecular and cellular biology laboratory equipment. Ability to problem solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision making.Ability to work effectively, both independently and collaboratively within a team.Technical competence with software programs such as Microsoft Office (Word, Excel, and PowerPoint) and scientific graphics packages (such as Origin) is strongly preferred. Ability to learn and apply software programs and applications as needed.Ability to perform various molecular biology techniques and muscle mechanics experiments.Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues, supervisors and customers face to face.Ability to follow through on assigned projects and provide timely follow-up as needed.Ability to meet consistent attendance.


Working conditions will require working on a muscle mechanics setup, working on a bench with molecular reagents and/or cardiac tissues, working with mice/other animal models/bacterial cells in the laboratory and in the animal facility, working in front of a computer for several hours, working outside standard working days or hours as required. The employee will work with frequent interruptions and distractions. There may be exposure to research specific chemicals and the employee will need to take Chemical Safety Training. Required personal protective equipment may include a standard lab coat, gloves and safety glasses when appropriate. May have exposure to hazards.

Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





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Job No:
Posted: 4/26/2021
Application Due: 7/25/2021
Work Type: Full Time