Job Title: Research Assistant 3
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8778
Job Description
POSITION OBJECTIVE

Working under limited supervision, coordinate research work in medical and related technical areas. This position will coordinate activities for laboratory research projects focusing on hematopoietic stem cell biology; specifically, within the development of new therapeutics. The individual will work closely with multi-investigator team consisting of the principal investigator, and undergraduate and medical students within the Comprehensive Cancer Center. This position will work with animals.

ESSENTIAL FUNCTIONS

Coordinate activities within the research team conducting experiments, manage experimental tasks, and resolve questions and correct scientific errors. These include characterizing the therapeutic benefit of specific inhibitors of 15-PGDH on bone marrow transplantation efficacy, and the chemoprotective effect of these inhibitors on marrow, GI, and other adult stem cell populations. (30%)Work closely with the principal investigator to design and perform experiments associated with additional models of hematopoiesis including elucidating mechanisms of hematopoietic biology during aging and bone marrow failure (20%)Carry out complex research assignments of a non-routine nature. Design and perform In vivo murine techniques. Specific experimental studies will require the ability to conduct bone marrow transplantation in mice, to isolate bone marrow stem cells from mice, to analyze bone marrow stem cells using FACS based methodology, and to conduct parallel studies involving isolation of human bone marrow stem cells, and to perform xeno-transplantation of human hematopoietic stem cells into immune deficient mice. Perform In vitro techniques including flow cytometry, PCR, cell culture, western blot, cell growth, etc. (15%)Devise new protocols and techniques for research projects involving a high degree of skill and training. Manage and design individual experiments, reporting these in a consistent fashion and in a timely manner, managing issues of the scientific method including positive and negative controls, reproducibility, sample size estimate valuation, trends and troubleshooting, and refining techniques into standard operating procedures in the laboratory. Careful documentation and accountability is required. (7%)Evaluate adequacy of techniques; study and test new procedures and analyze data. This will involve developing methodologies for BM and spleen RNA preparations post transplant for single cell RNA sequencing studies. Will work with principal investigator to analyze RNAseq data using bioinformatics software. (7%) May supervise students and technicians. This will involve training in basic methodology and supporting with studies when needed. (7%)Perform complex statistical analysis to be used in grant applications and manuscripts. May co-author research projects. (6%)

NONESSENTIAL FUNCTIONS

May monitor the laboratory budget and provide monthly updates to lab principle investigator.Provide input and recommendations to the principal investigator regarding budgetary matters. (5%)Perform other duties as assigned. (3%)

CONTACTS

Department: Continuous contact with Principal Investigator to provide weekly data updates as well as a small team within the laboratory working on similar research. Moderate contact with SPORE principle investigator to provide information on the status of research projects. Moderate contact with entire SPORE faculty team to update them on research project status. Moderate contact with the director of the NCRM to update on collaborative research.

University: Contact with the staff in the animal resource center to maintain proper animal husbandry/ address issues.

External: Little or no contact with external constituents.

Students: Contact with graduate students to assist in thesis projects and provide experimental support.

SUPERVISORY RESPONSIBILITY

May supervise students and technicians

QUALIFICATIONS

Experience: 3 to 5 years of related experience required.

Education/Licensing: Bachelor of Science degree required.

REQUIRED SKILLS

Experience with molecular biology techniques including PCR and western blotting.Experience handling and administering intraperitoneal injections on mice.Experience with flow cytometry and cell sorting.Experience with sterile technique and cell culture. Ability to work collaboratively in studies requiring coordination between multiple investigators.Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times. Ability to meet consistent attendance. Ability to interact with colleagues, supervisors, and customers face to face.

WORKING CONDITIONS

General laboratory environment. Employee will be exposed to blood-borne pathogens, chemicals, and radiation. Employee will need to wear appropriate protective equipment such as gloves, coat, and eyewear. Working conditions will require working at the bench in a molecular genetics/ biology laboratory, working with mice and other animal models in the laboratory and in the animal facility, and when needed working outside standard working days or hours as required by the needs of a given experiment.

Case's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

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In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

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Case Western Reserve University has implemented a COVID Vaccination policy requiring evidence of COVID-19 vaccination for all students, faculty and staff with an on-campus presence. Religious and medical exemptions may be provided in accordance with applicable federal and state laws. For more information go to: https://case.edu/equity/covid-19-accommodations. Applicants may contact the Office of Equity at 216-368-3066 /equity@case.edu for information regarding religious or medical exemptions as an accommodation.

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