Research Assistant 3
Job Title: Research Assistant 3
Location: Case Main Campus
Full/Part Time: Full-Time
Job ID: 8778
Job Description POSITION OBJECTIVE
- Coordinate activities within the research team conducting experiments, manage experimental tasks, and resolve questions and correct scientific errors. These include characterizing the therapeutic benefit of specific inhibitors of 15-PGDH on bone marrow transplantation efficacy, and the chemoprotective effect of these inhibitors on marrow, GI, and other adult stem cell populations. (30%)Work closely with the principal investigator to design and perform experiments associated with additional models of hematopoiesis including elucidating mechanisms of hematopoietic biology during aging and bone marrow failure (20%)Carry out complex research assignments of a non-routine nature. Design and perform In vivo murine techniques. Specific experimental studies will require the ability to conduct bone marrow transplantation in mice, to isolate bone marrow stem cells from mice, to analyze bone marrow stem cells using FACS based methodology, and to conduct parallel studies involving isolation of human bone marrow stem cells, and to perform xeno-transplantation of human hematopoietic stem cells into immune deficient mice. Perform In vitro techniques including flow cytometry, PCR, cell culture, western blot, cell growth, etc. (15%)Devise new protocols and techniques for research projects involving a high degree of skill and training. Manage and design individual experiments, reporting these in a consistent fashion and in a timely manner, managing issues of the scientific method including positive and negative controls, reproducibility, sample size estimate valuation, trends and troubleshooting, and refining techniques into standard operating procedures in the laboratory. Careful documentation and accountability is required. (7%)Evaluate adequacy of techniques; study and test new procedures and analyze data. This will involve developing methodologies for BM and spleen RNA preparations post transplant for single cell RNA sequencing studies. Will work with principal investigator to analyze RNAseq data using bioinformatics software. (7%) May supervise students and technicians. This will involve training in basic methodology and supporting with studies when needed. (7%)Perform complex statistical analysis to be used in grant applications and manuscripts. May co-author research projects. (6%)
- May monitor the laboratory budget and provide monthly updates to lab principle investigator.Provide input and recommendations to the principal investigator regarding budgetary matters. (5%)Perform other duties as assigned. (3%)
- Experience with molecular biology techniques including PCR and western blotting.Experience handling and administering intraperitoneal injections on mice.Experience with flow cytometry and cell sorting.Experience with sterile technique and cell culture. Ability to work collaboratively in studies requiring coordination between multiple investigators.Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times. Ability to meet consistent attendance. Ability to interact with colleagues, supervisors, and customers face to face.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.