Research Assistant 1

Job description

Job Title: Research Assistant 1
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8782

Working under moderate supervision, the Research Assistant 1 will perform research work to support the laboratory’s ongoing research of mechanisms of neurodevelopmental toxicity and neurotoxicants that disrupt lipid rafts. The research assistant will perform standard molecular techniques, Polymerase Chain Reaction, electrophoresis, RNA extraction, tissue-culturing/cell culture work, animal work, immunohistochemistry and general lab maintenance. The research assistant will also collect, verify, record and compile data. This position will work with animals.


    Perform research work using standard techniques of molecular biology/cell biology/biochemistry and cell culture including PCR, DNA analysis, protein analysis, retroviral/lentiviral construct, cloning/sub-cloning, histology, immunohistology and lipid raft isolation. (60%)Generate, maintain and analyze tissue culture cell lines from mice and rats for experimental purposes. Process tissue, isolate lipid rafts, perform immunohistochemistry, genotyping and analyze samples. Conduct cell-signaling assays and analyze western blots. Conduct neurite outgrowth assays and analyze results. (15%)Collect and record biochemical and histological experimental data. Organize raw data and update lab-related data folders. Collect and record results from cell culture experiments and provide data to laboratory members. Maintain clear and organized notebooks documenting procedures used and cell growth data generated. Maintain electronic records of data. Maintain accurate records/results and organized notes for experiments. (15%)Maintain test equipment utilized in experiments. Configure and operate laboratory instruments and ensure instruments are in working order. Perform routine lab maintenance including cleaning equipment and lab ware, dispose of biohazardous waste. Order laboratory supplies. Notify research assistant 2 to order reagents and other laboratory supplies when stock is low. Maintain chemical/biological inventory in compliance with electronic health system. (8%)


Perform other duties as assigned (2%)


Department: Daily contact with supervisor and lab members to discuss research and maintain workflow. Weekly contact with members of Division of Neonatology.

University: Occasional contact with other departments to share information and collaborate on projects.

External: Limited or no contact with vendors to exchange information.

Students: Occasional contact with student employees to explain policies and procedures.


This position has no direct supervision of staff employees.


Education/Experience: Bachelor’s degree and 0 to 1 year of laboratory experience or Associate’s degree in an approved biotechnology program and 0 to 2 years of experience required.


    Knowledge of commonly used concepts, practices and procedures within a particular field. Ability to rely on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment.Ability to meet consistent attendance. Ability to interact with colleagues, supervisors and customers face to face.Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Must be able and willing to learn new techniques, procedures, processes and computer gear to protect the health of the animals.Previous experience working with animals preferred. Strong cell biology skills with gel electrophoresis, Western blots and DNA synthesis.Strong organization skills and good habit of maintaining a clean lab working environment; demonstrate attention to detail and accuracy, time management skills and proven ability to successfully follow-through on assigned projects.Professional and effective verbal and written communication skills and good interpersonal skills with the ability to work and communicate with various individuals within and external to the University.Ability to work effectively independently and collaboratively within a team (must be highly motivated, responsible, dependable and a self-starter). Ability to work with sensitive information and maintain confidentiality.Proficiency in Microsoft Office is preferred. Experience using Adobe Photoshop and Image J is a plus. Must have the ability to maintain meticulous, complete and easily retrievable laboratory data.Must have the ability to willingly learn new techniques and procedures as needed, follow established protocols or laboratory procedures and request clarification if necessary.


General laboratory environment: The lab is an open floor plan with abundant bench space for animal handling and manipulations. Ample desk space with computers are also provided. May have exposure to hazards. May be required to use personal protective equipment, including standard lab coat, gloves and safety glasses when appropriate. The lab is equipped with one shared fume hood for storage of hazardous and non-hazardous materials. A common equipment room located adjacent to the lab are equipped with animal euthanization station. The cell culture room nearby is equipped with incubators, culture hood and microscope. Access to a multi-color Flow Cytometer and a Seahorse Analyzer belonging in a neighboring lab. The candidate should expect frequent interactions with lab members from that lab and must be willing to collaborate. Major physical demands include transferring animals between the lab and the animal holding facility, maintaining the animal colonies, as well as weekly changes of mouse/rat cages.

Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





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Job No:
Posted: 4/26/2021
Application Due: 7/25/2021
Work Type: Full Time