Research Assistant 2

Job description

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8774

Working under general supervision, the research assistant will plan and carry out research projects in accordance with general plans. The research projects are colorectal cancer biology and tumor microenvironment with the goals to define the role of cancer immune response and disease pathophysiology, and endoplasmic reticulum stress in leukemia microenvironment. This position works with animals, cell lines, animal and human specimens to study tumor immunobiology, signaling, and therapeutics.


    Plan and carry out project in accordance with general plans. Plan and carry out in vitro and in vivo animal experiments for various research projects. Conduct cell culture of established and primary cell lines, cell proliferation, clonogenic survival, DNA/RNA extraction, immunoblot, flow cytometry, PCR, maintain laboratory reagents including buffers and medias, and measure weight and tumor size in control and experimental mice. (50%)Design new protocols and techniques for research projects involving a high degree of skill and training, such as mouse xenograft tumor models and orthotopic PDX models (20%)Assist in developing procedures related to cancer biology and leukemia research, coordinate information with others as needed (15%)Train new staff and supervise students (8%)

    Oversee inventory of supplies necessary for daily laboratory work. Order reagents and other laboratory supplies (5%). Perform other duties as assigned. (2%)

Department: Daily contact with supervisor and other lab members.

University: Occasional contact with other departments to share information.

External: Occasional contact with vendors to exchange information.

Students: Moderate contact (up to 5 hrs/wk) with student employees.


May supervise research assistants, visiting students and scholars, and undergraduate students.


Education and experience: Bachelor's degree and 1 to 3 years of related experience or associate's degree in an approved biotechnology program and 2 to 4 years of experience.


    Has knowledge of commonly-used concepts, practices, and procedures within a particular field. Relies on instructions and pre-established guidelines to perform the functions of the job. Ability to meet consistent attendance.Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times. Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals. Previous experience working with animals preferred. Strong molecular biology skills (PCR, Western blots, Retroviral/Lentiviral constructs).Strong organizational skills and good habit of maintain a clean lab working environment; demonstrate attention to detail and accuracy, time management skills, and proven ability to successfully follow-through on assigned projects.Professional and effective verbal and written communication skills and good interpersonal skills with the ability to work and communicate with various individuals within and external to the University.Ability to work effectively independently and collaboratively within a team (must be highly motivated, responsible, dependable, and a self-starter). Proficiency in Microsoft Office and GraphPad Prism is preferred. Experience using Adobe Photoshop, Illustrator, and FlowJo is a plus.Must have the ability to maintain meticulous, complete, and easily retrievable laboratory data.Must have the ability to willingly learn new techniques and procedures as needed, follow established protocols or laboratory procedures, and request clarification if necessary. Ability to meet consistent attendance. Ability to interact with colleagues, supervisors, and customers face to face.

General laboratory environment. Mostly, will work at bench performing experiments that requires long time standing and sitting. Will perform some experiments in the animal room and standing at the laminar flow hood. Will work with mouse tissues including blood samples. Will work with archived formalin-fixed human tissues and established human fibroblasts and organoids. Will work with general lab chemicals. The employee may have to adjust working hours according to the nature of ongoing experiments and occasionally work extended hours to finish the experiments. Working on weekends and holidays are needed depending on the ongoing experiments and need to accelerate data generation and data collections.

Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the “Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals.” This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





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Job No:
Posted: 4/26/2021
Application Due: 7/25/2021
Work Type: Full Time