Description
A large research group at the Alzheimer’s Center at Temple is seeking an Assistant Scientist. The individual selected for this position will work in the subunit of the group that investigates the role of lipids and lipid metabolism proteins in neurodegeneration (Alzheimer’s disease and age-related macular degeneration) and will report to the leader of the lipid research subunit. The Assistant Scientist in the Alzheimer’s Center provides technical support by conducting experiments and independently handles experiments or small research projects requiring advanced knowledge and experience in a specific scientific area or discipline. Maintains records of data and may be involved in training graduate and medical students in research methods and procedures. 

Responsibilities include: maintaining immortalized and iPS cells, including medium change every 1-2 days, necessitating a medium change once over the weekend; maintaining the laboratory's animal colony; manipulating the genome of cell lines; conducting experiments including vector construction, iPS and immortalized cell genome editing using CRISPR/Cas9, transgene insertion into the genome of iPS and immortalized cells, directional differentiation of iPS cells to the desired cell types, characterization of the cell proteome and secretome using ELISA, western immunoblotting and immunohistochemistry and characterization of the cell transcriptome using quantitative PCR; analyzing human brain tissue specimens and animal (mouse and rat) brain, eye and other tissue, using ELISA, western immunoblotting, immunohistochemistry, quantitative PCR and staining with lipophilic reagents.

The Assistant Scientist receives extensive training in the requisite procedures and experimental protocols and will work independently, and is suitable for newer graduates. The Assistant Scientist also contributes to deriving new cell and animal models; maintains records including detailed outlines of employed procedures and experimental protocols, detailed records of conducted experiments and data, including a database of western, microscope and other types of images and a collection of spreadsheet files of numerical data; analyzes images using ImageJ and other image analysis software, analyze and graph numerical data using GraphPad Prism, prepare figures for presentation and present results at lab meetings and conferences. Performs other duties as assigned.

Required Education & Experience: BS degree or higher in a biology-related field and two years of research experience. An equivalent combination of education and experience may be considered.

Required Skills and Abilities:

Demonstrated ability to independently conduct various experimental techniques with trainingDemonstrated ability to maintain accurate research recordsWorking knowledge of applicable computer softwareFamiliarity and comfort with managing animal coloniesAbility to work independently and as part of the laboratory teamAbility to give direction to junior laboratory staff

This is a grant funded position

Temple University values diversity and is committed to equal opportunity for all persons regardless of age, color, disability, ethnicity, marital status, national origin, race, religion, sex, sexual orientation, veteran status or any other status protected by law. 

Compliance Statement: In the performance of their functions as detailed in the position description employees have an obligation to avoid ethical, legal, financial and other conflicts of interest to ensure that their actions and outside activities do not conflict with their primary employment responsibilities at the institution. Employees are also expected to understand and be in compliance with applicable laws, University and employment policies and regulations, including NCAA regulations for areas and departments which their essential functions cause them to interact. 

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Primary Location: Pennsylvania-Philadelphia-Health Sciences Campus (HSC)-Medical Education and Research Building
Job: Staff
Schedule: Full-time
Shift: Day Job
Employee Status: Regular