Life Science Technician II
Life Science Technician II🔍School of Medicine, Stanford, California, United StatesNew📁Research📅3 days ago Post Date📅89067 Requisition #This position is represented by SEIU Local 2007 and the collective bargaining agreement between the university and SEIU Local 2007 governs the terms and conditions of employment.The Konermann Lab in the Department of Biochemistry at the Stanford University School of Medicine is recruiting a Life Science Technician II (LST) to start as soon as possible.
The LST will immediately join a fast-moving project investigating human genetic risk in Alzheimer’s disease. The LST will collaborate closely with a senior graduate student and Professor Konermann to conduct high-throughput CRISPR transcriptional perturbation experiments in stem cell-derived neurons and glia. This project builds on recent high-profile work in genome-scale CRISPR screening (Konermann et al., Nature 2015), transcriptome engineering (Konermann et al., Cell 2018), and single-cell sequencing technologies.
- Maintenance and expansion of stem cell and stem cell-derived cultures.
- Generation of multiple brain cell types from human stem cells.
- Staining, sorting, transfection, transduction of cultured cells.
- Purification, cloning, quantification, sequencing of DNA/RNA.
About Silvana Konermann:
Dr. Silvana Konermann is an Assistant Professor in the Department of Biochemistry at the Stanford University School of Medicine, a Chan Zuckerberg Biohub Investigator, and an HHMI Hanna H. Gray Fellow. The focus of Dr. Konermann’s lab is the development and application of technologies for high-throughput transcriptional perturbations to understand the cellular and molecular pathways driving human genetic risk in neurodegenerative disease. Dr. Konermann received her B.S. from ETH Zurich and her Ph.D. in Neuroscience from MIT, where she was advised by Feng Zhang and received the international Harold M. Weintraub Graduate Student Award. She was a postdoc at the Salk Institute before joining Stanford as an Assistant Professor in 2019.
Keywords: CRISPR screening, neurodegeneration, Alzheimer’s, stem cells, single-cell sequencing. DESIRED QUALIFICATIONS:
- B.S. in Biology, Biochemistry, Neuroscience, Bioengineering, or a related field.
- At least 1 year of laboratory experience (including in college), incorporating strong hands-on experience with molecular biology and cell culture techniques.
- Bonus: experience working with sensitive cells (e.g. stem cells, primary neurons, etc.).
- Excellent communication, organization, and critical thinking skills.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.
- Ability to stand and move on hard surfaces for up to eight hours.
- Occasionally lift and handle materials up to 10 pounds.
- Rarely carry/pull and/or push up to 20 pounds.
- Must have correctible vision to perform duties of the job.
- Position may require repetitive motion.
WORKING CONDITIONS:Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required.
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu and the appropriate collective bargaining agreement at https://cardinalatwork.stanford.edu/hr-processes-policiespolicies/labor-relations-collective-bargaining.
- Schedule: Full-time
- Job Code: 5644
- Employee Status: Regular
- Grade: A24
- Department URL: http://biochem.stanford.edu/
- Requisition ID: 89067