Life Science Technician II (Fixed Term Ending 8/31/2022)

Job description

Life Science Technician II (Fixed Term Ending 8/31/2022)🔍School of Medicine, Stanford, California, United States📁Research📅Apr 06, 2021 Post Date📅89065 Requisition #This position is represented by SEIU Local 2007 and the collective bargaining agreement between the university and SEIU Local 2007 governs the terms and conditions of employment.

Baxter Laboratory is seeking a Life Science Technician II (LST II). This position will support the development of a highly scalable multiplexed serological assay for COVID-19. We will expand this assay to detect antibodies against different pathogens simultaneously. An additional goal is to expand multiplex testing to viral genomes and antigens.

  • Will perform experiments related to mass cytometry, immunofluorescence and sequencing.
  • Will be involved with collecting, storing, and processing SARS-CoV-2 samples.
  • This position requires the ability to troubleshoot experimental assays in conjunction with other Nolan lab members.
  • The LST II will maintain and manage assay stocks/reagents (e.g., preparation of buffers, blocking reagents, inventory of oligos, isotopes and antibodies).
  • Perform typical flow cytometry and microscopy techniques.
  • Perform cell culture and bacterial growth for protein synthesis.
  • Additionally, cell culture, cell count, RNA expression, protein quantification will periodically be required. Perform molecular biology protocols to verify conjugations (PCR, Bradford Stains, SDS-page gels).
  • Automation using robotics will consist a portion of the assay development.
  • Aid in data analysis processing using established software, ImageJ, and some basic R code.
  • The position includes maintaining detail records of performed experiments and their outcome. This is achieved in the form of a lab notebook where experiments and their results are recorded daily.


College coursework in Biology, Biochemistry, Bioengineering, Biophysics, or in a related discipline.

Extensive prior experience with single-cell mass cytometry, fluorescence-based flow cytometry, characterization of cell lines. Excellent laboratory management skills and knowledge of the full range of genomic, proteomic, biochemical and immunohistochemical laboratory techniques.

Familiarity with and adherence to laboratory safety best-practices and Stanford University Health and Safety policies and regulations.

Working closely on a team as a member and as a leader on specific research projects is essential, sharing data, training colleagues and trainees in new techniques and protocols, documenting research protocols and results and recording and analyzing data is key.

The candidate will also assist with preparation of data, figures and reporting for manuscripts, progress reports and grant submissions: Proficiency in English and strong writing skills are desired.


Two years of laboratory experience or two years of college coursework in the scientific discipline a combination of equivalent education and relevant work experience.


Work at the Life Science Technician II level requires experience and ability beyond that required for the Life Science Technician I, including increased technical skills and problem-solving abilities.


  • Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.
  • Ability to stand and move on hard surfaces for up to eight hours.
  • Occasionally lift and handle materials up to 10 pounds.
  • Rarely carry/pull and/or push up to 20 pounds.
  • Must have correctible vision to perform duties of the job.
  • Position may require repetitive motion.

* - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required.


  • Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
  • Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
  • Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, and the appropriate collective bargaining agreement at

Additional Information
  • Schedule: Full-time
  • Job Code: 5644
  • Employee Status: Fixed-Term
  • Grade: A24
  • Requisition ID: 89065




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Job No:
Posted: 4/8/2021
Application Due: 6/7/2021
Work Type: Full Time