Research Assistant 2

Job description

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8762
Job Description Position Objective

Working under the general supervision of the principal investigator and senior level staff within the laboratory, the Research Assistant 2 will plan and carry out experiments in accordance with research plans at the cellular and molecular levels using genetically engineered mice and cells isolated and grown from these animals. The research work of the lab is focused on identifying the cellular and molecular mechanisms underlying inflammatory skin disease and its associated co-morbidities including arthritis, depression, atherosclerosis and thrombosis. This position will work with mice.

Essential Functions

    Plan and carry out a series of experiments in accordance with a research study under the supervision of the principal investigator and senior members of the lab. Experiments will utilize isolated cells, fluids and collected tissues from mice and humans. Collect and analyze experimental data including ELISA, flow cytometry, in vitro bioassays and perform statistics and make graphs of the findings. Maintain complete, detailed, accurate and organized records and results of experiments, protocols, and use of animals. (40%)Conduct a series of experiments utilizing the following techniques: tissue-sectioning using a cryostat or microtome, immunohistochemistry, histochemistry, immunofluorescence staining of tissues, flow cytometry using cells isolated from tissues or blood. Perform microscopy, image capture and image analyses. Perform protein and RNA isolation and expression analyses. May assist in developing improved techniques, projection methods or procedures. (35%)Perform animal genotyping using PCR and perform animal husbandry to assist the animal husbandry manager when needed. (10%)Contribute to the day-to-day function of the lab, train new staff, direct the work of students. Oversee inventory, generate and clean up reagents used for experiments, and ensure the lab is clean. (10%).

NonEssential Functions

Perform other duties as assigned. (5%)

CONTACTS

Department: Daily contact with supervisor, technicians, senior lab members and lab manager to discuss research. Work closely with other research assistants, fellows and collaborating investigators. Contact with other members of the Nutrition Department and the Psoriasis Center for Research Translation (CORT).

University: Regular contact with other departments, including the Animal Resource Center and the university veterinary staff to share information. Occasional contact with other departments, including the IACUC to share information.

External: Contact with collaborators at CCF-LRI (CCLCM) at both research and administrative levels. Limited or no contact with vendors to exchange information.

Students: Contact with student employees.

Supervisory Responsibility

This position has no direct supervision of staff employees. Train new staff; direct the work of students.

QUALIFICATIONS

Education and Experience: Bachelor's degree and 1 to 3 years of related experience or Associate's degree in an approved biotechnology program and 2 to 4 years of experience required.

REQUIRED SKILLS

    Basic knowledge of commonly-used concepts, practices and procedures within the fields of biology, immunology, anatomy and physiology.Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to Animal Resource Center’s mission. Must handle animals with care and respect at all times.Effective oral and written communication skills and good interpersonal skills; ability to work and communicate with various individuals within and external to the university.Must have exceptional organizational skills and be capable of multitasking. Must demonstrate attention to detail and accuracy.Ability and willingness to rely on and follow instructions and pre-established guidelines to perform the functions of the job.Previous experience working with animals preferred. Must be willing to work with mice.Ability to operate laboratory equipment.Must be self-motivated, goal oriented, responsible and capable of working well in a team environment and capable of working on their own with daily supervisor.Good computer skills, including Microsoft Office and data analysis.Must demonstrate willingness to learn new techniques, procedures, processes and computer programs as needed.Ability to interact with colleagues, supervisors and customers face to face.Ability to meet consistent attendance.

Working Conditions

General laboratory environment. May have exposure to hazards including paraformaldehyde, human tissue and human cells, general chemicals and mice.

Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the “Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals.” This position and all animal research personnel are subject to internal compliance to ARC SOP’s and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

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Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.

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Job No:
Posted: 4/1/2021
Application Due: 5/31/2021
Work Type: Full Time
Salary: