Research Assistant I - Clayton Foundation Laboratories for Peptide Biology, Dr. Alan Saghatelian

Location
La Jolla, CA
Posted
Mar 31, 2021
Employment Type
Full Time
Institution Type
Four-Year Institution

Application Instructions:

Employment History Include your last ten (10) years of employment history, or length of your employment history if less, including all positions (even those that are not relevant to this position) and periods of unemployment. Incomplete information could disqualify you from further consideration.

POSITION SUMMARY

The Research Assistant I position is responsible for performing various molecular biology, cell and biochemistry techniques; as well as sample preparation for mass spectrometry (metabolomics and porteomics) in support of the Principal Investigator (PI) and lab research goals. Other duties include assisting with general laboratory maintenance, including equipment maintenance, and ordering of supplies, etc.

ESSENTIAL FUNCTIONS

(50%) Molecular and Cellular Biology, and Biochemistry Techniques

  • Molecular cloning (Standard methods and Gibson cloning)
  • Western blotting (Southern blotting optional, but desired)
  • Purification and manipulation of nucleic acids (e.g. RT-PCR, plasmid & genomic DNA preparation)
  • Ultracentrifugation
  • Culture of cell lines
  • Cryopreservation
  • Transfection
  • Lentiviral vector production

(40%)  Mass spectrometry sample preparation  

  • Tissue and cell processing for mass spectrometry
  • Immunoprecipitation experiments for mass spectrometry protein interactions
  • Assistance with data processing for metabolomics and proteomics experiments

(10%) Lab Support

  • Assist with cell maintenance.
  • Assist with preparing standard media and solutions.
  • Assist with weekly inventory of lab supplies.
  • Assist with stock and organize incoming lab supplies.
  • Clean glassware and equipment as needed.

EXPERIENCE

Required:

  • No professional experience required with relevant Bachelor’s degree or above.
  • Demonstrated experience and application of molecular biology knowledge preferably in biochemistry and cell culture.
  • Demonstrated experience with general lab maintenance, including cleaning, stocking supply inventory, and ensuring the maintenance of a sterile environment.

Preferred:

  • 2+ years of experience as a lab technician or undergraduate researcher.
  • Experience with mass spectrometry techniques.

EDUCATION

Required:

  • Bachelor’s degree in biological/chemical sciences or related field, or equivalent combination of education and work experience.   

SKILLS AND ABILITIES

Required:

  • Theoretical and hands on knowledge of tissue culture and molecular biology techniques.
  • Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions.
  • Ability to learn and carry out new experimental procedures with accuracy and efficiency. Demonstrated ability to follow verbal and/or written protocols, including follow-up on previously begun procedures.
  • Demonstrated ability to record data accurately and enter into appropriate medium.
  • Skill in the operation and maintenance of various lab equipment.
  • Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
  • Proficiency with common office software applications as well as software applications used for research, recording, and analysis.
  • Highly organized, effective at multitasking, adept at juggling changing priorities
  • Proven ability to work both independently and also as part of a collaborative team.
  • Excellent communication skills, both oral and written.
  • Strong interpersonal skills, including tact, diplomacy, and flexibility.
  • Demonstrated enthusiasm about research and ability to work as part of a team
  • Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.

SPECIAL CONDITIONS OF EMPLOYMENT

  • Must be willing to work in an animal-related research environment.
  • Must be willing to work an adjusted schedule to support research needs.
  • Successful completion of the Institute’s background investigation.
  • Willing to sign an employee confidentiality agreement.

PHYSICAL REQUIREMENTS

This position requires constant adjusting focus, seeing, sitting, touching/feeling, and working inside. 

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