The person in this position works together with the Principal Investigator, Mahasin Osman, to design and carry out research experiments related to the molecular and cellular basis of cancer health disparity and links to metabolic diseases (diabetes and brain degenerative disorders). Specifically we study several novel pathways involving the role of scaffold proteins in nuclear-cytoplasm crosstalk and ER-stress. Perform experiments, analyze data, prepare manuscripts for publication, train and manage laboratory personnel and students, and manage all aspects of the research laboratory. We employ a multifaceted approach, including super-resolution microscopy, color IHC and kinemics. We use cell culture, mouse models and human tissues. Other projects in the lab include drug discovery both targeted and unbiased screens. We collaborate widely with experts in the field.Qualifications:
Education/experience/licensingPH.D. (preferred) or MS degree & 2 years of research experience required. Background knowledge in molecular pathogenesis of disease. Molecular biology facility management. Must have experience in molecular biology techniques, including the maintenance of PCR thermal-cyclers, centrifuges, spectrophotometers, laminar flow hoods for PCR work, pipets, freezers, storage conditions for samples and reagents, and proper standard operating procedures for molecular biology laboratory. · Cell culture facility management. At least 1 year of experience managing cell culture facilities, including maintaining certification of laminar flow hoods of cell culture work, maintaining proper function of cell culture incubators, establishing proper standard operation procedures for the culture of both primary and immortal cell lines. · Experience in light microscopy and immunofluorescence staining on tissues, primary cell cultures and on immortal cell lines. Ability to learn advanced microscopy upon training · Experience in the maintenance and husbandry of transgenic mice and/or rats and in non-survival mouse and rat surgery for the isolation and culture of primary cell cultures. · Working knowledge of Microsoft Word, PowerPoint and Excel to include basic computer skills. · Computer database management with five years' experience establishing and maintaining computer databases for cell culture archives and experimental results, including proper computer backup. · Successful completion of NIH Protection of Human Subjects, CITI, HIPAA and laboratory safety training is required (may be completed post hire). Knowledge, Skills & Abilities · Ability to work independently and use discretion in selecting and adapting research protocols. · Ability to evaluate results at each step of an experiment and clearly communicate said results to the primary investigator. · Ability to effectively work as part of a research team. · Ability to locate, understand and keep abreast of relevant scientific literature. · Ability to contribute effectively to preparation of manuscripts and grant applications. · High communication skills both oral and written · Ability to help with training young graduate and undergraduate students in the labWorking Conditions:Works in a research laboratory environment. Must be familiar with chemical hygiene and biosafety procedures, and willing to accept training in such procedures. Work involves a very good exposure to and handling of research animals (mice/rats), potentially biohazardous substances (recombinant DNA, retroviruses, cultured human cell lines, human and mice samples and primary cells), and potentially hazardous chemicals (organic solvents, various drugs).The irregular nature of the timing of research projects may require some evening and weekend hours to complete experiments.The above list of duties is intended to describe the general nature and level of work performed by people assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the people so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct and control the work of employees under his/her supervision.
The above list of duties is intended to describe the general nature and level of work performed by people assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the people so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct and control the work of employees under his/her supervision.The University of Toledo is an equal opportunity, affirmative action employer. The University of Toledo does not discriminate in employment, educational programs, or activities on the basis of race, color, religion, sex, age, ancestry, national origin, sexual orientation, gender identity and expression, military or veteran status, disability, familial status, or political affiliation. The University of Toledo provides reasonable accommodation to individuals with disabilities. If you require accommodation to complete this application, or for testing or interviewing, please contact the Human Resources Department at 419-530-4747 between the hours of 8:30AM and 5:00PM or apply online for an accommodation request.Computer access is available at most public libraries and at the Office of Human Resources located in the Center for Administrative Support on the Main Campus of the University of Toledo.