University of Colorado Denver
Department of Neurology | Anschutz Medical Campus
Assistant Professor - Research
Job Code: 1303
Position Number: 00781352***This position is only open to CU Employees***OverviewThe Department of Neurology has an opening for a motivated full-time Assistant Professor - Research to lead projects investigating alphaherpesvirus and coronavirus biology, amyloid formation, and related RNA sequencing analysis in tissue culture cells, animal models (rhesus macaques and guinea pigs) and human samples. This position will collaborate with study colleagues at all levels of the organization. We are seeking an individual who is collaborative, communicative, caring, detail oriented and organized, proactive, able to think critically, focused on quality, dedicated to personal growth, and highly motivated in all areas of study coordination. The Assistant Professor will work independently and in collaboration with Dr. Maria Nagel who directs VZV and COVID research projects. He/she will design, performance and analysis of experiments; prepare manuscripts and grants, and direct activities of professional research assistants/postdoctoral fellows working on these projects.The position is located at the Anschutz Medical Campus. Hours are usually 8:00 to 5:00 weekdays, but occasional evening and frequent weekend hours may be required. We are the premier research university in Colorado, serving more than 30,000 undergraduate, graduate and health professions students in Denver, Aurora and online and awarding nearly 4,000 degrees each year. The renowned researchers and award-winning faculty offer more than 100-degree programs through 13 colleges and schools. In 2008, the University was awarded more than $371 million in research grants and contracts. The Anschutz Medical Campus includes over 5 million square feet of research, educational and clinical space on 227 acres.Examples of Work PerformedResponsibilities include, but are not limited to the following:
- Conceptualize/lead projects to investigate the role of alphaherpesviruses in amyloid-associated diseases including Alzheimer’s disease, diabetes, and macular degeneration.
- Conceptualize/lead projects to investigate the role of SAR-Co-V2 infection in human organoid cultures (retina and pancreas)
- Design experiments for motor neuron co-cultures with astrocytes and skeletal muscle with VZV infection; determine glutamate production, neuronal toxicity, and astrocyte activation.
- Assist and manage various components of program project life cycle that include:
- Assist with Project 1 of the PI’s current PO1 that investigates the role of VZV in giant cell arteritis; perform RNASeq experiments in temporal artery biopsy samples from diseased and normal individuals, analyze results using the Ingenuity Pathway Analysis (IPA) program and validate findings.
- Assist with PO1 Alzheimer's disease-related supplemental projects by determining amyloidogenic environment in SVV-infected rhesus macaque CSF and serum.
- Ph.D. in neuroscience or related field
- Postdoctoral Fellowship in the fields of neuroscience and alphaherpesvirus pathogenesis.
- A minimum of 3 years’ experience in (1) alphaherpesvirology research; (2) non-human primate models of SVV infection; (3) whole brain dissection.
- Experience writing grants and serving as a co-investigator/Principal Investigator on NIH-funded grants that result in successfully funded grants
- Experience in BSL3 laboratories, SARS-CoV-2 experimental approaches
- Proficient in Microsoft Office Suite (e.g., Word, Excel, PowerPoint), Sigmaplot or equivalent statistical analysis program; Axiovision imaging software; Image J.
- Expertise in neurokinin-1R signaling, viral contributions to amyloidosis, bioinformatics, RNASeq analysis and Ingenuity Pathway analysis.
- Experience in immunohisto- and cyto-chemistry, confocal microscopy and imaging software (Axiovision and Image J), basic tissue culture and virology techniques (infection, titers, stock preps), primary human neuron cultures, brain dissection and hippocampal slice cultures, and virus-infection in animal models (particularly rodents and non-human primates).
- NIH grant assembly
- Histology, tissue/cell culture of primary human cells including neurons; alphaherpesvirus handling, expansion and titering; nucleic acid extraction, RT-PCR and qPCR; protein analysis; microscopy; RNA sequencing experiments and IPA software; siRNA experiments and primer design; handling of human and animal tissue; brain dissection; amyloid and electron microscopy analysis; handling, drawing blood and inoculating guinea pigs intravenously with virus.
- Outstanding interpersonal and communication skills with the ability to work independently and within diverse team structures.
- Respect patient privacy/rights and put patient safety first, excellent patient rapport for any clinically-related projects or human sample acquisition
- A positive, motivated, engaged, and caring attitude
- Great attention to detail and quality
- Ability to work with colleagues and patients in a confidential and professional manner
- Follow best practices in research (ICH guidelines, GCP, FDA guidelines)
- Ability to follow and execute a study protocol and proactively prevent deviations
- Ability to follow direction of the PI and direct supervisor, follow all department SOPs and department policies
- Good documentation practices
- Dedication to professional development and life-long learning
- Ability to be adaptable, flexible, and to think on your feet
- Experience with web-based tracking systems and Microsoft Office suite (e.g., Excel, Word, PowerPoint and Access)
Job Category: Faculty
Primary Location: Aurora
Posting Date: Feb 26, 2021
Unposting Date: Ongoing