RESEARCH TECHNICIAN II - ASOKAN LAB DEPT OF SURGERY (PRODUCTION)
School of Medicine:
Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.
Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
The Asokan laboratory in the Department of Surgery is focused on gene therapy research. Ongoing efforts include advancing viral vector development through our expertise in molecular biology and scalable recombinant virus production capabilities. We are currently seeking to hire a skilled research technician to assist in the production of viral vectors for different gene therapy applications. A competitive salary and benefits package will be provided according to the Duke University School of Medicine guidelines.
The candidate will work closely with the AAV production team and is expected to independently perform daily activities in the laboratory, which include:
- Cell culture maintenance
- Standard molecular biology techniques (e.g. maxi preps, transfections, PCR, RNA and DNA extraction, molecular cloning, gel electrophoresis etc.)
- Preparation of culture media, chemicals, buffers, reagents and solutions
- Operate laboratory equipment such as incubators, ultracentrifuges, chromatography and other specialized equipment utilized for virus production
- Follow SOPs, document protocols and methods, compile and graph data
- Maintain sufficient inventory of material, supplies and equipment for vector production
- Routinely clean and maintain laboratory equipment
- Maintain database of different plasmids and viral vector preparations
Work generally requires a Bachelor’s degree in biology, microbiology or biomedical engineering or related scientific field. Completed Bachelor's degree is strongly preferred.
Prior cell culture and protein/DNA purification experience is strongly preferred.
Experience with molecular cloning techniques is preferred.
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