Senior Professional Research Assistant
Job description
Description
University of Colorado School of Medicine
University of Colorado | CU Anschutz Medical Campus
School of Medicine, Department of Pediatrics
Section of Hematology Oncology Bone Marrow Transplantation
Senior Professional Research Assistant (SPRA)
Position #00703776– Requisition #20203
* Applications are accepted electronically ONLY at www.cu.edu/cu-careers *The University of Colorado Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment. We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.The Department of Pediatrics, Section of Hematology Oncology Bone Marrow Transplantation has an opening for a full-time Senior Professional Research Assistant position. Nature of Work:- Laboratory-based translational research on the immunology of cancer and hematopoietic stem cell biology, with a focus on bone marrow transplantation.
- Conduct experiments; assist with project planning, data interpretation, training scientists, students, and volunteers; as well as, authorship of presentations and publications.
- Seeking a motivated and experienced scientist who has the initiative and skill-set to take charge on in vivo projects, specifically murine models, and is well versed with molecular lab techniques, from start (designing) to finish (expression in cells), and is willing and able to mentor students and new lab members.
- Culturing of human hematopoietic stem cells, embryonic stem cells (ES)/induced pluripotent stem cells (iPS) and differentiation of these cells into innate immune cells 15%Multicolor Flow cytometry (FACS) 10%Extensive murine model work under the scope of cancer and cancer-predisposition models 15%
- Conducting experiments using techniques including, qPCR, cloning, transfection/transduction, protein/RNA extraction, single cell sequencing, immunofluorescence microscopic cell imaging 15%
- Incucyte cytotoxicity assays 10%
- Preparing data for discussion and presentation 10%
- Attending and presenting at lab meetings 10%
- Assisting with lab organization and upkeep 10%
- Assisting in or producing publications 5%
Qualifications
Minimum Qualifications:
- Master’s degree in a biological science field or related field + 10 years of professional laboratory experienceExtensive murine experience including, but not limited to: maintenance of colonies; injections and implantations; tissue and bone marrow harvesting. Must be able to independently design murine studies from start to finishPrior experience mentoring students/staff
- Must be willing and able to pass a national criminal background check
- PhD degree in Genetics or related field 10+ color flow cytometry experiencePrevious experience in presenting and/or aiding in publicationsCompetency with Flowjo, Microsoft office, Prism Graphpad, and analysis software(s) in Immunohistochemistry
- Good verbal and written communications in English
- Ability to stay organized under pressure
- Knowledgeable of professional laboratory protocols
- Ability to complete lab functions involving cell culture and aseptic techniques
- Competency with molecular biology techniques
- Ability to complete live cell binding assays
- Animal experience (designing/mouse handling/injections/harvesting/sacrificing)
- Background in immunology and stem cell biology
- Experience handing human blood products, specifically stem cells
- Ability to troubleshoot and independently resolve technical issues with experiments
- Can independently construct primers and manipulate plasmid constructs; transform and isolate plasmid DNA; and utilize said DNA for lentiviral transductions of both primary cells and cell lines
- Cell culture and maintenance of various cell lines
- Ability to perform 5+ color flow cytometry
Job Category: Faculty
Primary Location: Aurora
Schedule: Full-time
Posting Date: Feb 18, 2021
Unposting Date: Feb 24, 2021, 1:59:00 AM