RESEARCH TECHNICIAN II
School of Medicine:
Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.
Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
The Duke Functional Genomics Shared Resource is seeking a Lab Technician II to support activities in the areas of high-throughput CRISPR screening, chemical screening and cell line engineering. Position will be for approximately one year with extensions contingent upon funding, performance, and needs of Functional Genomics Shared Resource. Duties: · Interact with users of the Duke Functional Genomics Shared Resource to assist on projects, develop assays, perform experiments, analyze data and provide instrument training. · Develop and execute protocols for cell line engineering of primary and cancer-derived cells using CRISPR, RNAi and ORF technology. · Perform experiments including transfections, transductions, plate reader assays, high-content screening, PCR, immunofluorescence and cloning. · Assist in facility operations, including scheduling of projects and instrument usage, maintenance of lab stocks and respond to inquiries for facility services. · Review scientific literature to remain current with new developments in relevant areas, including genome editing and functional genomics. The successful candidate will be highly organized, detail-oriented and self-motivated. Knowledge of cell culture and molecular biology techniques or lab automation is desirable but willingness and ability to learn is essential.Minimum Qualifications
Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
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