Research Assistant 2
Job Title: Research Assistant 2
Location: Case Main Campus
Full/Part Time: Full-Time
Job ID: 8663
Job Description POSITION OBJECTIVE
- Plan research projects in accordance with general plans. Carry out experimental tasks, and resolve questions, and correct scientific errors. These include characterizing the therapeutic benefit of specific inhibitors of 15-PGDH on bone marrow transplantation efficacy, and the chemoprotective effect of these inhibitors on marrow, GI, and other adult stem cell populations. (35%)Conduct a series of experiments to supply scientific information as part of a larger project. Perform in vivo murine techniques. Conduct bone marrow transplantation in mice, to isolate bone marrow stem cells from mice, to analyze bone marrow stem cells using FACS based methodology, and to conduct parallel studies involving isolation of human bone marrow stem cells, and to perform xeno-transplantation of human hematopoietic stem cells into immune deficient mice. Perform In vitro techniques including flow cytometry, PCR, cell culture, western blot, cell growth, etc. (25%)Collect and analyze data. Maintain careful and detailed documentation. Perform complex statistical analysis to be used in grant applications and manuscripts. Provide reports in a consistent fashion and in a timely manner. (17%)Manage issues of the scientific method including positive and negative controls, reproducibility, sample size estimate valuation, trends and troubleshooting, Refine techniques into standard operating procedures in the laboratory. Maintain primary and established cell lines. (14%)
- Oversee and manage/order supply inventory. (3%)May train new staff; direct the work of students. (3%)Perform other duties as assigned. (3%)
- Has knowledge of commonly-used concepts, practices, and procedures with molecular biology techniques including PCR and western blotting.Has knowledge of commonly-used concepts, practices, and procedures with handling and administering intraperitoneal injections on mice.Has knowledge of commonly-used concepts, practices, and procedures with flow cytometry and cell sorting.Has knowledge of commonly-used concepts, practices, and procedures with sterile technique and cell culture. Ability to work collaboratively in studies requiring coordination between multiple investigators.Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times. Ability to meet consistent attendance. Ability to interact with colleagues, supervisors, and customers face to face.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.