Research Assistant 2

Job description

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8663

Working under general supervision, plan and carry out research projects in accordance with general plans. The research projects focus on hematopoietic stem cell biology; specifically, within the development of new therapeutics. This position will work with animals.


    Plan research projects in accordance with general plans. Carry out experimental tasks, and resolve questions, and correct scientific errors. These include characterizing the therapeutic benefit of specific inhibitors of 15-PGDH on bone marrow transplantation efficacy, and the chemoprotective effect of these inhibitors on marrow, GI, and other adult stem cell populations. (35%)Conduct a series of experiments to supply scientific information as part of a larger project. Perform in vivo murine techniques. Conduct bone marrow transplantation in mice, to isolate bone marrow stem cells from mice, to analyze bone marrow stem cells using FACS based methodology, and to conduct parallel studies involving isolation of human bone marrow stem cells, and to perform xeno-transplantation of human hematopoietic stem cells into immune deficient mice. Perform In vitro techniques including flow cytometry, PCR, cell culture, western blot, cell growth, etc. (25%)Collect and analyze data. Maintain careful and detailed documentation. Perform complex statistical analysis to be used in grant applications and manuscripts. Provide reports in a consistent fashion and in a timely manner. (17%)Manage issues of the scientific method including positive and negative controls, reproducibility, sample size estimate valuation, trends and troubleshooting, Refine techniques into standard operating procedures in the laboratory. Maintain primary and established cell lines. (14%)


    Oversee and manage/order supply inventory. (3%)May train new staff; direct the work of students. (3%)Perform other duties as assigned. (3%)


Department: Continuous contact with senior research associate to provide weekly data updates as well as a small team within the laboratory working on similar research. Moderate contact with SPORE principle investigator to provide information on the status of research projects. Moderate contact with entire SPORE faculty team to update them on research project status. Moderate contact with the director of the NCRM to update on collaborative research.

University: Contact with the staff in the animal resource center to maintain proper animal husbandry/ address issues.

External: Little or no contact with external constituents.

Students: Contact with graduate students to assist in thesis projects and provide experimental support.


This position has no direct supervision of staff employees. May train new staff; direct the work of students.


Education/Experience: Bachelor’s degree and 1 to 3 years of related experience; OR Associate’s degree in an approved biotechnology program and 2 to 4 years of experience required.


    Has knowledge of commonly-used concepts, practices, and procedures with molecular biology techniques including PCR and western blotting.Has knowledge of commonly-used concepts, practices, and procedures with handling and administering intraperitoneal injections on mice.Has knowledge of commonly-used concepts, practices, and procedures with flow cytometry and cell sorting.Has knowledge of commonly-used concepts, practices, and procedures with sterile technique and cell culture. Ability to work collaboratively in studies requiring coordination between multiple investigators.Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times. Ability to meet consistent attendance. Ability to interact with colleagues, supervisors, and customers face to face.


General laboratory environment. Employee will be exposed to blood-borne pathogens, chemicals, and radiation. Employee will need to wear appropriate protective equipment such as gloves, coat, and eyewear. Working conditions will require working at the bench in a molecular genetics/ biology laboratory, working with mice and other animal models in the laboratory and in the animal facility, and when needed working outside standard working days or hours as required by the needs of a given experiment.

Case's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





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Job No:
Posted: 2/18/2021
Application Due: 5/19/2021
Work Type: Full Time