RESEARCH TECHNICIAN III - NEUROBIOLOGY
School of Medicine:
Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.
Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
Perform a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of Duke University research operations.
- Perform a variety of complex technical duties involved in conducting physical, chemical, biological and other research laboratory tests, experiments and determinations to obtain data for research purposes; compile, analyze, document and draw tentative conclusions from experimental results.
- Execute and troubleshoot all facets of mouse model of epilepsy. This includes performing stereotaxic surgery for placement of injection cannulas, recording electrodes, and viruses; constructing novel recording electrode array on headmounts with preamplifiers; deploying novel computer software for detection of seizures using EEG and video data; performing repeated intravenous injections into retroorbital sinus of adult mice.
- Assume responsibility of ordering supplies and equipment for lab members, specifically aligning the order with proper grant code. Place and track orders at the request of lab member. Maintain sufficient inventory of material, supplies and equipment for performance of duties; clean and maintain laboratory equipment. Set up instruments, material and apparatus and operate laboratory equipment required for specific tests. Notify lab members of lab meetings and presenters at lab meetings on weekly basis.
- Serve as point person for interaction with DLAR and assure laboratory procedures are conducted in accord with IACUC approved protocol. Prepare amendments to IACUC protocol as needed.
- Confer with principal investigator to review work assignments and develop plans for research experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of the particular equipment as required. Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
- Conduct biochemical assays including Elisa, Fluorescent resonant energy transfer, Western blotting. Prepare and maintain primary cultures of rat and mouse cortical neurons and glia. Prepare culture media, chemicals, reagents and solutions. Obtain, prepare, mount and stain tissue for microscopic examination.
- Perform other related duties incidental to the work described herein.
The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.
Work generally requires skills acquired through the completion of a bachelor's degree in neurobiology, chemistry, biology, or similar science related scientific field.
Work requires two years of experience in a research laboratory. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.
Interested candidates should send a CV and three references to Dr. McNamara ([email protected]).
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