Research Assistant 2
Job Title: Research Assistant 2
Location: Case Main Campus
Full/Part Time: Full-Time
Job ID: 8682
Job Description POSITION OBJECTIVE
- Plan and carry out projects in accordance with general plans; isolate, purify and characterize primary neutrophils, macrophages, eosinophils and mast cells from mice of different genetic strains. (20%)May assist in developing improved techniques, projection methods or procedures. Maintain primary cell cultures of the different leukocyte cell types. Maintain established hematopoietic cell lines (murine and human). Make and maintain diverse stock solutions of cell culture media used for the studies. (20%) Manage animal resources used in experiments by keeping detailed records of animal inventory, maintain mouse breeding colonies, setting up and checking breeding pairs for litters and separating cages at time of weaning. (15%)Conduct a series of experiments to supply scientific information as part of a larger project. These experiments will characterize the intracellular signaling pathways that mediate different modes of regulated inflammatory cell death and release of inflammatory cytokines by neutrophils, macrophages, eosinophils and mast cells. The experimental assays will involve: (a) basic molecular biology methods (plasmid purification and characterization, transfection of mammalian cells with cDNA expression vectors); (b) basic cell biological methods (cell fractionation, preparation of cell extracts, measurement of basic cell function such as intracellular levels of ions); and (c) basic biochemistry (immune precipitation, enzyme-linked immunoassays, gel electrophoresis, Western and Northern blotting and a various types of chromatography). (20%)Collect, organize and analyze research data for presentation in laboratory meetings. (15%)
- Oversee inventory and standard operating procedures, maintain lab records. Maintain equipment, prepare buffer/media/reagents and dispose biohazardous waste. (5%)Train new staff; direct the work of students. (3%)Perform other duties as assigned. (2%)
- Knowledge of commonly used concepts, practices and procedures in chemistry, physics, biochemistry and molecular/cellular biology. Prior experience with cell culture and basic biochemical and cell biological methods. Previous experience working with small lab animals preferred, but training will be available for individuals without prior animal use training. Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Ability to follow instructions and pre-established guidelines to perform the functions of the job with minimal supervision.Strong organization skills; ability to maintain a laboratory notebook, keep careful records and write research reports with attention to scientific detail.Ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to effectively operate basic molecular and cellular biology laboratory equipment. Ability to problem solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision making.Ability to work effectively, both independently and collaboratively within a team.Technical competence with software programs such as Microsoft Office (Word, Excel and PowerPoint) and scientific graphics packages (such as Prism) is strongly preferred. Ability to learn and apply software programs and applications as needed.Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues, supervisors and customers face to face.Ability to follow through on assigned projects and provide timely follow-up as needed.Ability to meet consistent attendance.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.