Job description

School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Research Scientist / Lab Manager

The Barkauskas lab seeks a highly motivated, organized, and enthusiastic scientist, preferably with previous experience in lung biology, to work as a research scientist and lab manager. The Barkauskas lab is a basic and translational science lab that seeks to better understanding how the lung normally repairs after injury and how these processes may be disarrayed in diseases like pulmonary fibrosis. The lab’s specific area of expertise is in lung epithelial stem cell biology and Dr. Barkauskas has pioneered some of the techniques used to study lung biology in 3- dimensional organoid culture systems.

The candidate will participate in all aspects of the research process, including (but not limited to) investigation, testing/validation of results, reporting of findings, and planning of experiments with other lab members. The candidate will perform routine experimental protocols and procedures to support the objectives of laboratory research projects. The candidate will assist in preparation of data for publication and presentation and scholarly meetings. The candidate may assist in supervision of more junior lab members.


• Performs experimental protocols and procedures and can assist in the design of new or unusual protocols or techniques. Protocols include (but are not limited to) tissue processing, immunofluorescence staining, imaging, set up and maintenance of murine organoid cultures, molecular biology techniques (cloning, PCR, DNA and protein extraction).

• Works with human lung samples: includes isolation of cell types from dissociated tissue, banking of cells, establishment of human organoid culture systems, and set up and maintenance of precision cut lung slice culture (PCLS).

• Keeps accurate and detailed records of experiments and results and is willing to assist in troubleshooting of unexpected results.

• Assists in preparation of data for publication or presentation at scientific meetings.

• Maintains stocks of general lab materials and places orders for equipment and supplies.

• Assists with lab maintenance responsibilities.

• Responsible for formulating, comprehending, and following a large number of Standard Operating Procedures (SOPs) and participating in inspections and regulatory oversight.

• Assists with maintenance of mouse lines specific to the Barkauskas lab, including participation in design of rodent breeding programs.

• Performs animal work including injections, tissue harvest, and survival surgeries.

Minimum Qualifications


Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.


Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE


A qualified candidate will have an advanced degree (Master’s or PhD) in biology, zoology or another directly related scientific field. A candidate with a bachelor’s degree but with significant relevant experience will be considered. Experience in lung biology (or other epithelial stem cell biology) is preferred.

Additional experience with immunology, flow cytometry, advanced microscopy, or organoid cell culture is a plus.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.




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Job No:
Posted: 1/9/2021
Application Due: 1/26/2021
Work Type: Full Time