Research Assistant 3

Job description

Job Title: Research Assistant 3
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8550

Working under limited supervision, the Research Assistant 3 will coordinate research activities and carry out complex research projects focusing on discovering and characterizing novel interactions between HIV and host cell proteins that modulate infection process. The research project will investigate molecular mechanisms that cells employ to sense and inhibit viral infection and HIV proteins that antagonize cellular defenses, using virologic, biochemical and molecular genetic approaches. This position will work closely with the principal investigator to carry out the most complex assignments and coordinate research activities and experiments.


    Working closely with the principal investigator, carry out complex research assignments of a non-routine nature aimed to identify and characterize functional interactions between HIV and cellular proteins. (35%)Coordinate research activities; design, construct and produce retro- and lentiviral vectors using innovative cloning techniques for their use in complex molecular/biochemical experiments aimed to identify and characterize novel interactions between viral and cellular proteins, and for gene transfer into a broad range of indicator cell lines. Experiments will be performed in both established and primary human cells under BL2 and BL2+ containment conditions. (35%).Devise new protocols and techniques and utilize newly developed research techniques, as appropriate. Evaluate adequacy of techniques and protocols for non-routine biochemical experiments including, but not limited to, those designed to purify complexes between viral and cellular proteins for their subsequent proteomic analyses. Perform downstream characterization of high priority proteomic hits and other relevant targets using appropriate biochemical methods. May co-author research projects. May supervise students and technicians. (15%)Maintain accurate and thorough records of the obtained research data. May monitor the laboratory budget. (10%).


Perform other duties as assigned (5%).


Department: Daily contact with the principal investigator, research associates and students within the research group to coordinate and in the course of conducting research activities.

University: Occasional contact with principal investigators or laboratory personnel in other research groups or core facilities to exchange expertise or information.

External: Occasional contact with sales representatives for product inquiries or to arrange services.

Students: Regular contact with students in the laboratory to direct the work and/or make adjustments to procedures.


May supervise students and technicians.


Experience: 3 to 5 years of research laboratory experience.

Education/Licensing: Bachelor’s degree in biochemistry, molecular biology, virology or related discipline required.


    Practical knowledge and understanding of commonly used concepts, practices and procedures within the biochemistry, molecular-genetics and/or virology fields. Must possess the following basic technical skills: sterile technique; reagent and media preparation, cell culture, DNA transfection, preparation of plasmid DNA, molecular cloning, immunoprecipitation, western blotting, PCR, biochemical purification techniques and/or production of retroviral/lentiviral vectors.Must be highly motivated, conscientious and willing to learn.Professional and effective verbal and written communication skills. Strong organization skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy, time management skills and follow-through. Ability to work effectively independently and collaboratively within a team. Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making.Must be compliant with applicable regulatory guidelines.Ability to operate complex laboratory equipment including, flow cytometer and QPCR.Familiarity with standard laboratory safety policies and procedures.Proficiency in Microsoft Office. Must demonstrate willingness and ability to learn new techniques, procedures, processes and computer programs as needed.Ability to meet consistent attendance. Ability to interact with colleagues, supervisors and customers face to face.


The employee will be working in a laboratory in the Department of Molecular Biology and Microbiology. Experiments using retroviral vectors and viruses will be carried out under BSL2/BSL2+ conditions. There are no unusual hazards; possible exposure to the usual hazards in the form of general chemicals, infectious agents, bloodborne pathogens, paraformaldehyde, phenol, chloroform, methanol, ethidium bromide, ultraviolet light, sonication and potentially, to radioisotopes. Required personal protective equipment includes a standard lab coat, gloves and safety glasses when appropriate. The employee will need to be able to lift up to 25 lbs, bend/stoop, kneel, crouch, push, pull and reach above shoulders. The employee will use repetitive motion, including the use of a keyboard and computer mouse while performing job duties.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





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Job No:
Posted: 11/13/2020
Application Due: 2/11/2021
Work Type: