Research Assistant 2

Job description

Job Title: Research Assistant 2
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8555

Working under general supervision, the Research Assistant 2 will plan and carry out molecular biology and biochemistry, maintain mammalian/insect cell lines, as well as express and purify proteins. The research assistant will also perform laboratory maintenance duties, including buffer, media, gel and reagent preparation and disposal of bio-hazardous waste. Although not necessarily handling live viruses directly, this position is expected to be able to work in a shared Biosafety Level 2+ (BSL2+) tissue culture facility, for example, to prepare large-scale cell culture for virus infection.

The research interest of the lab is focused on structure-guided antiviral drug discovery and vaccine design. The laboratory uses a wide variety of techniques including cryo-electron microscopy (cryoEM), virology, molecular biology and cellular biology.


    Plan and carry out general molecular biology projects, including design and implement cloning strategies, PCR, mutagenesis, restriction enzyme digestion and analysis, gel electrophoresis, gel purification, ligation, cloning, plasmid preparation, bacterial transformation, bacterial culturing, DNA purification, sequencing, sequence analysis, recombination and RNA preparation. Perform calculations involving molarity, percent solutions and serial dilutions. (40%).Conduct a series of experiments to supply scientific information as part of a larger project. Perform transfect or infect mammalian and insect cells with expression vectors. (30%)Prepare large-scale cell culture in a BSL2+ facility. Maintain cell cultures including mammalian, insect and bacterial cells. Perform small to medium scale protein production and purification. (10%)Prepare reagents and under limited supervision, conduct experiments, collect and analyze data, and evaluate experimental results. (10%)


    Prepare, operate and trouble shoot equipment in experimental procedures. Oversee inventory. (5%)Perform other duties as assigned. (5%)


Department: Daily contact with faculty, postdoctoral scholars and investigators to discuss research, research education, direction, goals and time management. Frequent contact with laboratory employees in collaboration in common research project.

University: Occasional contact with staff and faculty as needed to share information. Utilize core university facilities for experimentation and maintain strong relationship with core mangers for research collaborations.

External: Limited contact with sales representatives for product inquiries.

Students: Occasional contact with students as needed to share information.


This position has no direct supervision of staff employees. Train new staff; direct the work of students.


Education/Experience: Bachelor’s degree in the Life Sciences or equivalent in a related field and 1 to 3 years of related research experience or Associate’s degree in an approved biotechnology program and 2 to 4 years of experience required.


    Basic knowledge of molecular and cellular biology.Familiarity with protein expression and purification is a strong plus.Experience with BSL2 or BSL2+ viruses is a plus. Follow instructions and pre-established guidelines to perform the functions of the job. Ability to operate molecular and cellular biology laboratory equipment.Ability to work in a biosafety level 2+ facility after training.Effective verbal and communication skills.Ability to interact with colleagues, supervisors and customers face to face.Ability to work effectively independently and within a team.Good organization skills, ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to follow-through on assigned projects and provide timely follow-up as needed.Ability to rely on instructions and pre-established guidelines to perform the functions of the job. Ability to meet consistent attendance.


Laboratory is located in a designated space within the School of Medicine. There are frequent interruptions and distractions. Exposure to research specific chemicals. Laboratory apparel might be required including lab coat, gloves and safety glasses when appropriate. Some work is expected to be done in a shared Biosafety Level 2+ (BSL2+) facility. Biosafety training will be provided.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.





Diversity Profile: University



View more

Learn more on Inside Higher Ed's College Page for University

Arrow pointing right
Job No:
Posted: 11/13/2020
Application Due: 2/11/2021
Work Type: