Research Assistant 2
Job Title: Research Assistant 2
Location: Case Main Campus
Full/Part Time: Full-Time
Job ID: 8555
Job Description POSITION OBJECTIVE
- Plan and carry out general molecular biology projects, including design and implement cloning strategies, PCR, mutagenesis, restriction enzyme digestion and analysis, gel electrophoresis, gel purification, ligation, cloning, plasmid preparation, bacterial transformation, bacterial culturing, DNA purification, sequencing, sequence analysis, recombination and RNA preparation. Perform calculations involving molarity, percent solutions and serial dilutions. (40%).Conduct a series of experiments to supply scientific information as part of a larger project. Perform transfect or infect mammalian and insect cells with expression vectors. (30%)Prepare large-scale cell culture in a BSL2+ facility. Maintain cell cultures including mammalian, insect and bacterial cells. Perform small to medium scale protein production and purification. (10%)Prepare reagents and under limited supervision, conduct experiments, collect and analyze data, and evaluate experimental results. (10%)
- Prepare, operate and trouble shoot equipment in experimental procedures. Oversee inventory. (5%)Perform other duties as assigned. (5%)
- Basic knowledge of molecular and cellular biology.Familiarity with protein expression and purification is a strong plus.Experience with BSL2 or BSL2+ viruses is a plus. Follow instructions and pre-established guidelines to perform the functions of the job. Ability to operate molecular and cellular biology laboratory equipment.Ability to work in a biosafety level 2+ facility after training.Effective verbal and communication skills.Ability to interact with colleagues, supervisors and customers face to face.Ability to work effectively independently and within a team.Good organization skills, ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to follow-through on assigned projects and provide timely follow-up as needed.Ability to rely on instructions and pre-established guidelines to perform the functions of the job. Ability to meet consistent attendance.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.