Research Assistant 2
- Employer
- Case Western Reserve University
- Location
- Case Main Campus
View more
- Administrative Jobs
- Academic Affairs, Research Staff & Technicians
- Employment Type
- Full Time
- Institution Type
- Four-Year Institution
Job Details
Location: Case Main Campus
Regular/Temporary: Regular
Full/Part Time: Full-Time
Job ID: 8555
Job Description POSITION OBJECTIVEWorking under general supervision, the Research Assistant 2 will plan and carry out molecular biology and biochemistry, maintain mammalian/insect cell lines, as well as express and purify proteins. The research assistant will also perform laboratory maintenance duties, including buffer, media, gel and reagent preparation and disposal of bio-hazardous waste. Although not necessarily handling live viruses directly, this position is expected to be able to work in a shared Biosafety Level 2+ (BSL2+) tissue culture facility, for example, to prepare large-scale cell culture for virus infection. The research interest of the lab is focused on structure-guided antiviral drug discovery and vaccine design. The laboratory uses a wide variety of techniques including cryo-electron microscopy (cryoEM), virology, molecular biology and cellular biology. ESSENTIAL FUNCTIONS
- Plan and carry out general molecular biology projects, including design and implement cloning strategies, PCR, mutagenesis, restriction enzyme digestion and analysis, gel electrophoresis, gel purification, ligation, cloning, plasmid preparation, bacterial transformation, bacterial culturing, DNA purification, sequencing, sequence analysis, recombination and RNA preparation. Perform calculations involving molarity, percent solutions and serial dilutions. (40%).Conduct a series of experiments to supply scientific information as part of a larger project. Perform transfect or infect mammalian and insect cells with expression vectors. (30%)Prepare large-scale cell culture in a BSL2+ facility. Maintain cell cultures including mammalian, insect and bacterial cells. Perform small to medium scale protein production and purification. (10%)Prepare reagents and under limited supervision, conduct experiments, collect and analyze data, and evaluate experimental results. (10%)
- Prepare, operate and trouble shoot equipment in experimental procedures. Oversee inventory. (5%)Perform other duties as assigned. (5%)
- Basic knowledge of molecular and cellular biology.Familiarity with protein expression and purification is a strong plus.Experience with BSL2 or BSL2+ viruses is a plus. Follow instructions and pre-established guidelines to perform the functions of the job. Ability to operate molecular and cellular biology laboratory equipment.Ability to work in a biosafety level 2+ facility after training.Effective verbal and communication skills.Ability to interact with colleagues, supervisors and customers face to face.Ability to work effectively independently and within a team.Good organization skills, ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.Ability to follow-through on assigned projects and provide timely follow-up as needed.Ability to rely on instructions and pre-established guidelines to perform the functions of the job. Ability to meet consistent attendance.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
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Reasonable AccommodationsCase Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.
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Organization
Working at Case Western Reserve University
Case Western Reserve University is among the nation's leading research institutions. Founded in 1826 and shaped by the unique merger of the Case Institute of Technology and Western Reserve University, Case Western Reserve is distinguished by its strengths in education, research, service and experiential learning. Located in Cleveland's University Circle, we offer nationally recognized programs in the arts and sciences, dental medicine, engineering, law, management, medicine, nursing and social sciences.
Student enrollment exceeds 9,800 students, forty percent of whom are undergraduates. Facultyand students hail from more than 90 countries, with academic interests that reach every region of the world. Case Western Reserve, with the support of individuals, corporations and foundations, aids nearly 100 designated research centers.
As a service-oriented institution dedicated to civic leadership, Case Western Reserve seeks individually and collectively to prepare our students to improve the human condition and to direct the benefits of discovery toward a better society. This effort is not limited to the university's classrooms, laboratories, libraries, residence halls and athletic fields, but includes partnerships with many other institutions. We build these partnerships believing that our ability to improve the human condition should begin in our own community.
Case Western Reserve University remains Ohio's top-ranked school among the nation's premier national universities, as ranked by U.S. News & World Report and is the only Ohio institution ranked in the top 50 nationally. We promote a culture of inquiry marked by rigor, creativity, curiosity, innovation, respect, sensitivity and open communication of ideas.
The university supports interdisciplinary partnerships in education and research with numerous faculty holding joint appointments in more than one Case Western Reserve school or department, as well as at neighboring institutions. These relationships launched many of the unique pairings of science, business and liberal arts into the centers and programs that enhance the undergraduate and graduate experience at the university. Our dual degree programs enrich the educational experience for those students who choose to balance the technical requirements of engineering or the sciences with a strong interest in the humanities.
With more than $375 million in research funding annually, the university attracts outstanding undergraduate and graduate students along with renowned faculty whose research has made significant contributions to the way we live and work. Case Western Reserve counts 15 Nobel laureates among our alumni and current and former faculty, including the first American scientist to ever receive the prize.
Our students' experiences in cultural institutions, clinics, social service agencies and industry are not viewed as “extra-curricular,” but create the learning experience that defines a Case Western Reserve education. The university's service programs in the Cleveland Municipal School District have impacted more than 145,000 students with more than 43 signature programs. The university has launched more than 580 community partnerships on the local, national and international levels. Annually the university sponsors Case for Community Day, an event where hundreds of Case Western Reserve employees and students lend their time and talent to targeted community development projects in the Greater Cleveland area.
Our alumni number more than 110,000 and constitute a “who's who” of every profession. Alumni represent one of the university's strongest resources, playing a vital role in campus life through their work as advisors, mentors, friends and advocates.
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