BIOL - Norambuena Lab Tech 2

Job description

Job location: Charlottesville, VA


Employment Type: Full-time
Posted data: 2020-11-09
Req: R0019660

The Department of Biology at the University of Virginia is seeking a Laboratory Tech. to work in the laboratory of Dr. Andrés Norambuena to study a novel molecular mechanism regulating mitochondrial function and its potential role in Alzheimer’s disease (EMBO J. 2018;37(22). pii: e100241). The successful candidate will work in a laboratory that mainly focuses in understanding molecular mechanisms disrupted by amyloid beta oligomers and Tau and its relationship to brain energy metabolism. Our laboratory’s interests are mainly basic, but we are also interested in applying our knowledge in future translational activities towards generating novel targets that will help our efforts to combat Alzheimer’s disease. Dr. Norambuena’ laboratory has broad interest in Cell signaling & Metabolism, Membrane Trafficking and Disease Mechanisms. We are also working closely with world-class experts in live mouse brain metabolic imaging. The lab tech. will primarily be responsible for overseeing the mouse colony. This includes mouse colony breeding and maintenance, genotyping, maintaining the animal protocol, working closely with the PI and lab personnel to manage the overall colony size, and may be asked to assist lab personnel with surgical procedures.

Other lab specific duties are described below.

The incumbent will attend weekly meetings with the Principal Investigator to discuss experimental results, conduct literature searches, and present results in lab meetings. The incumbent will work under supervision but independently and assist other members of the lab. This position also includes support activities such as preparation of reagents for the laboratory, management of safety training and records. The ideal candidate will be a highly-motivated individual with attention to detail. This position is reviewed on a yearly basis and its continuation is dependent on funding and satisfactory performance.

Review of applications will begin immediately, but the position will remain open until filled.

Duties and responsibilities will be determined by Andrés Norambuena and include:

Lab duties:

1. Ordering through Marketplace, ordering and picking up from Molmart, and restocking from Chemistry storeroom.

2. Picking up packages and put items away and collecting packing slips.

3. Restocking cell culture room with pipettes and tubes.

4. Autoclaving H2O for tissue culture room.

5. Refilling and autoclaving pipettes.

6. Making buffers, stock reagents, plasmid cDNA, cell growth media and solutions, and assisting other lab members with experiments

7. Washing laboratory glassware when necessary.8. Keep public laboratory notes.

Mice husbandry

1. Setting up breading pairs for wilde-type, hTau and/or TG2576 mice (or any other transgenic mice that might became relevant for our project) needed for experiments in collaboration with Dr. Ammasi Periasamy at the Keck Center for Cellular Imaging and Dr Song Hu at Washington University in St. Louis.

2. Weaning pups

3. Tattooing (to distinguish individual mice from each other)

4. Genotyping hTau mice. These mice have been genetically engineered to express human versions of the protein, tau. For technical reasons, we must breed mice that contain only 1 copy of the human tau gene, and these matings yield, on average, pups of the following genotypes: 25% with 2 copies of the human tau gene, 50% with 1 copy of the human tau gene and 25% without any human tau gene. Because our actual experiments must also be done with mice carrying 0 or 1 copy of the human tau gene, we must genotype all the pups. Similar genotyping will be required for Tg2576 or any other transgenic mice that might became relevant for our project.

APPLICATION PROCESS:

Process for External Applicants: Please apply through Workday, and search for “BIOL - Norambuena Lab Tech 2”. Complete an application online and attach a cover letter, CV/resume, and contact information for three professional references (name, email address, telephone number, and address).


Process for Internal UVA Applicants: Please apply through your Workday Home page, search “Find Jobs”, and search for “BIOL - Norambuena Lab Tech 2”. Complete an application online and attach a cover letter, CV/resume, and contact information for three professional references (name, email address, telephone number, and address).

For questions about this position or the application process please contact Rich Haverstrom, Faculty Search Advisor, at rkh6j@virginia.edu.

The University of Virginia, including the UVA Health System and the University Physician’s Group are fundamentally committed to the diversity of our faculty and staff. We believe diversity is excellence expressing itself through every person's perspectives and lived experiences. We are equal opportunity and affirmative action employers. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender identity, marital status, national or ethnic origin, political affiliation, race, religion, sex (including pregnancy), sexual orientation, veteran status, and family medical or genetic information.

The anticipated salary range for the position is $18.00 - $19.00/hr.

This full-time position will remain open until filled.

MINIMUM REQUIREMENTS

Education: Associates degree required. A BA or BS in Biology, Biochemistry, or other related field is preferred.

Experience: Some-up to 4 years. At least one year of relevant laboratory experience in the above-mentioned duties is preferred. Experience as a student would be considered. Experience working with mice is highly preferred.

Licensure: None

PHYSICAL DEMANDS

This position can spend extensive periods of time standing and squatting while conducting experiments or caring for animals. Also requires keyboarding and other bench work and animal procedures requiring finer finger manipulation.

The University of Virginia, including the UVA Health System which represents the UVA Medical Center, Schools of Medicine and Nursing, UVA Physician’s Group and the Claude Moore Health Sciences Library, are fundamentally committed to the diversity of our faculty and staff. We believe diversity is excellence expressing itself through every person's perspectives and lived experiences. We are equal opportunity and affirmative action employers. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender identity or expression, marital status, national or ethnic origin, political affiliation, race, religion, sex (including pregnancy), sexual orientation, veteran status, and family medical or genetic information.

 

 

 

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Job No:
Posted: 11/10/2020
Application Due: 2/8/2021
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