Postdoctoral Fellow, Immunobiology and Microbial Pathogenesis, Dr. Daniel Hollern

The laboratory of Dr. Daniel Hollern at the Salk Institute of Biological Studies Department of Immunobiology and Microbial Pathogenesis in La Jolla, California seeks to recruit a Postdoctoral Fellow to engage in immuno-oncology research.

The main focus of the lab is to understand the immunological and molecular landscape of tumors with the goal of leveraging the immune component to promote anti-tumor immunity. In more detail, the lab is particularly interested in developing these approaches through the study of tumor infiltrating B cells and T cells. These studies will be pursued in syngeneic, orthotopic and genetically engineered mouse models as well as patient specimens.  The lab utilizes a multidisciplinary approach blending biologically appropriate models, molecular biology, and genomic analytical techniques including single cell and spatial transcriptomics and proteomics to interrogate pertinent questions in a number of in vitro and in vivo translational studies of breast cancer.

Position Highlights:

• Be involved in exciting projects to understand B cell and T cell immunobiology in solid cancers and studies exploring novel immunotherapeutic strategies.
• Conduct independent and collaborative studies in an Immunology lab focusing on clinically-relevant translational studies.
• Opportunity to learn techniques in single-cell and spatial computational genomics and immuno-oncology by projects investigating beneficial patient outcomes and anti-tumor immunity.
• Opportunity to develop new models for immune-oncology research.
• Participation at scientific meetings, membership in relevant scientific organizations, and applying for training grants will be encouraged and supported.

Ideal Candidate:

• Experience with analysis of genomic datasets such as RNA-seq, spatial datasets and single cell sequencing.
• Experience working with lab animals and expertise in the following cellular biology techniques:
• Murine syngeneic and genetically engineered models of cancers as well as allografts/xenografts
• Multi-parameter flow cytometry
• Cell culture
• Elispot or Luminex-based assays for cytokine/chemokine assessments.
• Immunofluorescent staining, imaging and fluorescent microscopy.
• Experience with standard molecular biology assays and genome editing (Crispr).
• Experience with proven record of publications in reputable journals, preferred.

Responsibilities:

• Perform investigational studies to understand the tumor microenvironment.
• Execute experiments independently and contribute to collaborative projects.
• Write grants.
• Write manuscripts for publishing.
• Train and supervise junior staff and participate in overall lab management.
• Analyze and present data at local and national meetings.

Credentials and Qualifications:

Ph.D. in Immunology, Cellular/Molecular Biology, or related fields. M.D. are welcome to apply. Hands-on-experience in laboratory research and good computer skills are required.