Biological Scientist II - Farrer Genetics Lab
Job no: 514880
Work type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Agriculture/Life Science/Vet Medicine, Research/Scientific/Grants
Department:29180300 - MD-NEUROLOGY-MOVEMENT DISORDER
Biological Scientist IIJob Description:
This full-time staff scientist position is based in the Laboratory of Neurogenetics and Neuroscience which is physically located within the McKnight Brain Institute and the Department of Neurology. The research is at the cutting edge of neuroscience modeling, and for several novel gene discoveries. The position is supervised by Dr. Matt Farrer-a leading neurogeneticist and genome scientist. Essential functions of this position include DNA extraction from human and mouse tissues, primer design and PCR, performing a variety of related molecular biology and cell culture tasks, performing a variety of related molecular biology and cell culture tasks, and conducting human genetic research for the affiliated Center for Human Genetics.
- DNA extraction from human and mouse tissues, primer design and PCR, for which familiarity with nucleotide databases such as the UCSD genome browser, NCBI blast and Ensembl, and related softwares will be required. The applicant would assist in the design, modification, cloning and characterization of recombinant plasmids, setting up the PCR, sizing the fragments on agarose, and using restriction ligation or TOPO-cloning into Gateway vectors. The resulting plasmids must be cultured in transformed E.coli, prior to plasmid isolation. Quality control will include Sanger (ABI3500) sequencing and transfection into immortalized mammalian cells (e.g. HEK293, PC12), prior to confirmation of expression by RT-PCR and western blotting.
- Performs a variety of related molecular biology and cell culture tasks. Examples include plasmid mutagenesis, using mismatched oligonucleotides; the propagation and purification of adeno-associated virus, to the subsequent transduction of primary neurons. Prior experience in bacterial and cell culture techniques is essential. Advanced training will be provided in molecular techniques, as required.
- Performs related human genetic research for the affiliated Center for Human Genetics including genomic library preparation for high-throughput sequencing, and large and small scale genotyping studies. The incumbent is expected to perform as part of a team, and may also train visiting/junior staff including graduate students involved in research.
- Contributes to general lab maintenance including ordering lab supplies and maintaining equipment/facility records and plasmid databases. Other miscellaneous duties include attendance at lab meetings, and reading research literature to stay contemporary with methodologic and technical advances to enhance the success of the team.
$39,000 - $45,000; commensurate education and experience
Pay Range Based on Market:
Lower reference point: $33,800 Midpoint: $43,900 Upper Reference point: $54,000Minimum Requirements:
Bachelor's degree in biology or a closely related field and three years of relevant experience; or a Master's degree in biology or a closely related field and one year of relevant experience; Doctoral degree in biology or a closely related field.Preferred Qualifications:
Doctoral Degree in a Biological or Health Sciences field or Master’s or Bachelor’s of Science degree with a strong background and practical experience in the relevant laboratory techniques for this position.
The preferred candidate’s skills include:
- Works in an organized and efficient manner while managing multiple laboratory experiments simultaneously
- Understands genomics, molecular and cell biology, and has interest in translational disease research in neurology
- The ability to work independently, analyze and present data, multitask, and maintain close communication with the Supervisor/Director, Staff, Postdoctoral Fellows and graduate students
- Excellent teamwork and communication skills. Positivity and optimism; a ‘can-do’ attitude, creativity and enthusiasm to get the work done, and with urgency.
In order to be considered, you must upload your cover letter and resume.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.Health Assessment Required:Yes
Advertised: 19 Oct 2020 Eastern Daylight Time
Applications close: 30 Oct 2020 Eastern Daylight Time