Biological Scientist II - Pediatrics Cellular and Molecular Therapy

Location
Main Campus (Gainesville, FL)
Posted
Oct 21, 2020
Institution Type
Four-Year Institution
Biological Scientist II - Pediatrics Cellular and Molecular Therapy

Job no: 514907
Work type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Agriculture/Life Science/Vet Medicine, Research/Scientific/Grants
Department:29092300 - MD-PEDS-CELL / MOLEC THERP DIV

Classification Title:

Biological Scientist III - Pediatrics Cell/Molecular Therapy

Job Description:

The Department of Pediatrics, Division of Cellular and Molecular Therapy is seeking candidates with experience in gene therapy research for a Biological Scientist II position in the laboratory of Dr. Arun Srivastava. This Biological Scientist position is in a research lab studying adeno-associated virus (AAV). The incumbent is expected to be able to 1. Modify protocols to improve existing techniques or to incorporate new diagnostic procedures; 2. Perform the execution assigned experiments; 3. Review and interpret scientific results; 4. Participate in the management of a rodent colony to achieve research objectives; 5. Conduct rodent procedures as necessary to complete research objectives 6. Present researching findings to PI and at laboratory meetings; 7. Maintain laboratory safety programs and train personnel in laboratory safety techniques; 8. Participate in the writing of grants, papers, and statements of work.

The Biological Scientist II must be able to perform the following essential functions:

Histology Analysis: Collecting organs and other tissue and embedding the specimens for sectioning either with a microtome or cryotome (for frozen sections). Following preparation of slides with sectioned tissue, staining with standard histology stains such as H&E, as well as with immunohistochemistry will be done. Participating in the image capture and analysis of tissue following intervention studies. This includes treating mice, collecting tissue and processing it for analysis.

Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines. Participating in in vitro studies exploring novel vectors in various cells. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.

Animal Studies: Handling mice for setting up timed matings, weaning animals and maintaining colonies. Participating in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injections. Other duties with the mice include biodistribution and gene expression studies.

Other: Performing various tasks as assigned.

Expected Salary:

$35,000 - $45,000 annually; commensurate within this range based on qualifications and experience.

Pay Range Based on Market:

(Annualized)

Lower reference point: $33,800 Midpoint: $43,900 Upper Reference point: $54,000

Minimum Requirements:

Bachelor’s degree in biology or a closely related field and three years of relevant experience ;or a Master’s degree in biology or a closely related field and one year of relevant experience; Doctoral degree in biology or a closely related field.

Preferred Qualifications:
  • Experience in gene therapy research (specifically AAV)
  • Animal colony management and handling of rodents experience
  • Advanced degree in related scientific field
  • Working knowledge of basic laboratory techniques
  • Extremely knowledgeable in laboratory procedures and be meticulous in recording, tracking & reporting of data collected
Special Instructions to Applicants:

To be considered for this position, please upload your cover letter or letter of interest, resume, and a list of professional references.

Monday through Friday, 8:00am to 5:00pm. Nights & weekends as needed for on-going research.

This is a time-limited position.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:Yes

Advertised: 19 Oct 2020 Eastern Daylight Time
Applications close: 26 Oct 2020 Eastern Daylight Time

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