Professional Research Assistant
University of Colorado – School of Medicine
Department of Surgery, Division of Urology
Professional Research Assistant
The University of ColoradoDenver l Anschutz Medical Campus seeks individuals with demonstrated commitmentto creating an inclusive learning and working environment. We value theability to engage effectively with students, faculty and staff of diversebackgrounds.
The University of Colorado Denver | Anschutz Medical Campus is a public research university serving more than 18,000 students. We award nearly 4,000 degrees each year, including more graduate and professional degrees than any other Colorado institution. With our solid academic reputation, award-winning faculty and renowned researchers, we offer more than 140 highly rated degree programs through 13 schools and colleges. The university receives over $400 million in research awards each year. In addition to the wide array of health-related programs and facilities offered at CU Anschutz in Aurora, Colo. a significant number of undergraduate and graduate degree programs are taught at CU Denver, our comprehensive campus in the heart of downtown Denver—one of America’s most vibrant urban centers. CU Denver is located steps from the Denver Center for Performing Arts and the LoDo District affording our students, faculty and staff access to a broad array of academic, professional, community, recreational and cultural outlets.
A national leader in health care and life sciences, CU Anschutz is the only comprehensive academic health sciences center in Colorado and the largest in the Rocky Mountain region. Located in Aurora, Colo., it is one of the newest education, research and patient care facilities in the world. With six schools and colleges, CU Anschutz trains the health sciences workforce of the future by blending education, research and clinical care all in one place. This strategic collaboration among diverse health care fields allows knowledge to travel directly from the research bench to the patient’s bedside. Read CU Anschutz Quick Facts here.
Overall Description of Responsibility or Nature of Work:
The incumbent will be part of the research team studying the mechanisms of neurogenic bladder dysfunction due to demyelination of the neural pathways innervating the lower urinary tract, as well as epigenetic changes in bladder sensory and spinal neurons. Research in the lab utilizes state-of-the-art technologies, including patch-clamp recordings in primary smooth muscle cell cultures and human cell lines, bladder innervating sensory and motor neurons, in vitro contractility studies, and pharmacogenetics in combination with in vivo urodynamic assessment of bladder function.Specific Position Duties with Percentages of Time:
- Investigate molecular mechanisms of neurogenic bladder dysfunction due to demyelination in the central nervous system using a mouse model of coronavirus-induced multiple sclerosis.
- Learn a novel in vivo pharmacological approach using DREADDs for silencing bladder nociceptive neurons via AAV-driven delivery of hM4Di receptors Perform experiments involving nucleic acid and protein isolation, RT-PCR and qPCR, Western blotting, use of adenoviral vectors Prepare data for presentations and publicationsAttend weekly lab meetings, and individual meetings with the mentorAdditional experiments will test the role of epigenetic changes in a number of nociception-related genes modulated by transcriptional complexes such as the Methyl-CpG-binding protein 2.
- BA or BSc with at least 1 year of laboratory research experience
- At least 2 years of laboratory research experience
- Experience in mutagenesis; tissue-specific transgenic vectors; qRT-PCR, Western Blot; in vivo Co-immunoprecipitation; Confocal microscopy, FACS, cell culture, transfections; generation of KO mice, genotyping.
- Co-authored publications in peer-reviewed biomedical journals
- Experience with optogenetic approaches, and in vivo electrophysiology
Job Category: Faculty
Primary Location: Aurora
Posting Date: Oct 16, 2020
Unposting Date: Ongoing