RESEARCH TECHNICIAN III
School of Medicine:
Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.
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A protective HIV-1 vaccine is major need for halting the current AIDS pandemic. Broadly neutralizing antibodies can protect againstHIV-1 infection in animal models. The Saunders laboratory investigates the antibody response to broadly neutralizing epitopes on HIV-1 Envelope protein in the context of Envelope vaccination. Many of the broadly neutralizing epitopes involve N-linked glycosylation, which is a central focus of the Saunders laboratory’s research. The candidate will work as part of a team to develop and evaluate novel vaccine concepts for HIV-1.
- Work independently to express protein-based vaccine candidates for in vitro characterization and in vivoimmunogenicity studies.
- Characterize vaccine immunogenicity with immunoassays including, flow cytometry, ELISA, and biolayer interferometry.
- Enable vaccine production and evaluation by performing the following experimental activities:
- Plasmid sequence analysis for on-goingprotein production needs.
- Large scale antibody and HIV-1 envelope production and purification.
- Large scale cell culture.
- Analysis of recombinant antibodies and HIV-1 envelopes using biochemical and immunological techniques, such as luminex multiplex bead binding, FPLC, SDS-PAGE, We stern blots, ELISA, biolayer interferometry.
- Molecular biology assays such as PCR, site-directed mutagenesis, DNA preparation.
- Manage protein receipt and distribution fromthe protein inventory
- Train laboratory staff and DHVI trainees.
- Compliantly order materials and reagents for ongoing projects in the lab.
- Maintain a detailed and accurate laboratory notebook.
- Perform preventative maintenance on laboratory equipment to ensure proper function.
- Perform other related duties as necessary andrequested.
Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program. A Master’s degree in biological sciences, chemistry, or a related field is highly preferred
Work requires two years of research experience. A related master's OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.
Department experience preference
A minimum of two years of biological laboratory experience including basic molecular and cellular biology techniques such as molecular cloning, PCR, DNA mutagenesis, and tissue culture
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