Research Assistant 4
Job Title: Research Assistant 4
Location: Case Main Campus
Full/Part Time: Full-Time
Job ID: 8498
Job Description POSITION OBJECTIVE
- Coordinate major research activities and staff. A major project is to generate human monoclonal antibodies from anti-specific B cells isolated from individuals exposed to malaria or SARS-CoV-2 and to characterize their function. This will involve skills in flow cytometry and molecular biology. Test, catalog and store samples. Evaluate adequacy of techniques; study and test new procedures and organize and analyze subsequent genotyping data. Assist the principle investigator to ensure all lab-related activities are completed in a timely manner and all protocols and regulatory guidelines are followed. (35%).Perform the most complex quantitative analytical procedures. Extract and measure DNA extraction using molecular biology techniques. Design primers and validate their utility using native DNA PAGE, PCR and qPCR amplification. Samples will need to be set up for analysis in the university sequencing facility. Clone the genes encoding variable regions of human immunoglobulins into genes expressing heavy chain regions of different IgG subclasses. (15%)To refine and develop assays that measure functional antibody responses to SARS-CoV-2 (e.g. neutralizing assays and those that measure antibody-dependent killing of virally infected cells (15%)Provide input and recommendations to principle investigator regarding significant developments in research projects. Provide updates and recommendations to principle investigator based on developments in the lab and staff performance. Assess analyses/procedures undertaken and whether subsequent changes may be needed; report recommendations directly to principle investigator. (10%)
- Assist in developing procedure and coordinate information with others. Devise new protocols and techniques for research projects involving a high degree of skill and training. Improve our ability to generate human monoclonal antibodies using other or improved procedures, such and EBV transformation of B cells prior to screening for production of antibodies to the antigens of interest. (5%)Co-author research projects. Write up results and methods for publication related to novel lab assays and international research studies. Assist in writing grant applications. (5%)Teach or instruct others in research techniques. Teach lab staff and/or students in laboratory techniques utilized and identified in research protocols. Teach evaluation and alignment of immunoglobulin sequences to determine their clonal origin or degree of somatic mutations relative to germline. Teach/advise how to analyze and present these data. (5%)May supervise Research Assistants and Technicians. Assist in the supervision of lab staff and assign weekly or monthly assignments; assure weekly progress reports are available for lab meetings. (5%)Maintain meticulous records (including for international receipt and shipping of materials and samples and regulatory entities). (3%)Attend the Center’s weekly scientific session. May present lab results at GHID or other seminars. (2%)
- Has solid knowledge of commonly used concepts, practices, and procedures within the field of molecular and microbiology with emphasis on parasitology.Has some facility with flow cytometry and basic concepts of immunology, especially humoral immunologyFacility with Microsoft Office applications, especially, Excel, Word and PowerPoint. While not required, familiarity and capabilities in bioinformatics will be useful.Relies on instructions and pre-established guidelines to perform the functions of the job. Ability to operate laboratory equipment to prepare buffers, perform PCR or qPCR, polyacrylamide and agarose electrophoresis.Professional and effective verbal and written communication skills with evidence of this provided.Attention to detail, good record keeping, accuracy, time management skills and follow-through.Ability to meet consistent attendance.Ability to interact with colleagues, supervisors, and customers face to face.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.